REGULATION OF ALGINATE PRODUCTION IN THE BACTERIUM PSEUDOMONAS AERUGINOSA

该子项目是利用NIH/NCRR资助的中心赠款提供的资源的许多研究子项目之一。子项目和研究者（PI）可能从另一个NIH来源获得主要资金，因此可以在其他CRISP条目中表示。所列机构为中心，不一定是研究者所在机构。我们已经确定Prc作为海藻酸盐生产的调节剂，并正在努力表征其作用。 我们认为Prc是一种蛋白酶，其作用是降解称为MucA的藻酸盐生产的关键调节剂。我们刚刚提交了一份手稿，描述了Prc的鉴定及其在调节藻酸盐生产中的作用的部分表征。我们正在继续我们的研究Prc充分表征其调节藻酸盐生产的机制。我们还开始研究鉴定和表征我们认为通过降解MucA来调节藻酸盐生产的其他蛋白酶。我们已经在细菌的基因组中确定了这些额外蛋白酶的三个候选者。 我们正在开始实验，以确定我们的候选人之一是否真的在调节藻酸盐生产中发挥作用。这些实验将使我们能够了解如何通过降解MucA在强毒株中诱导藻酸盐的产生。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. We have identified Prc as a regulator of alginate production and are working to characterize its role. We believe that Prc is a protease that acts to degrade a key regulator of alginate production called MucA. We have just submitted a manuscript for publication that describes the identification of Prc and a partial characterization of its role in regulation alginate production. We are continuing our studies of Prc to fully characterize the mechanism by which it regulates alginate production. We have also begun studies to identify and characterize additional proteases that we believe act to regulate to regulate alginate production by degrading MucA. We have identified three candidates for these additional proteases in the bacterium's genome. We are beginning experiments to determine if one of our candidates actually does play a role in regulating alginate production. These experiments will enable us to understand how the production of alginate in induced in virulent strains by the degradation of MucA.