REGULATION OF ALGINATE PRODUCTION IN THE BACTERIUM PSEUDOMONAS AERUGINOSA

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 我们已经确定PRC是藻酸盐生产的调节者，并正在努力确定其作用。我们认为PRC是一种蛋白酶，它能降解藻酸盐生产的关键调节因子粘液A。我们刚刚提交了一份手稿，描述了PRC的鉴定及其在海藻酸盐生产调控中的部分作用。我们正在继续我们对PRC的研究，以充分描述它调节藻酸盐生产的机制。我们也已经开始研究，以确定和表征更多的蛋白酶，我们认为这些酶通过降解粘液A来调节藻酸盐的生产。我们已经确定了细菌基因组中这些额外的蛋白酶的三个候选者。我们正在开始实验，以确定我们的候选者是否真的在调节藻酸盐生产方面发挥了作用。这些实验将使我们能够了解在强毒菌株中如何通过降解粘液A来诱导藻酸盐的产生。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. We have identified Prc as a regulator of alginate production and are working to characterize its role. We believe that Prc is a protease that acts to degrade a key regulator of alginate production called MucA. We have just submitted a manuscript for publication that describes the identification of Prc and a partial characterization of its role in regulation alginate production. We are continuing our studies of Prc to fully characterize the mechanism by which it regulates alginate production. We have also begun studies to identify and characterize additional proteases that we believe act to regulate to regulate alginate production by degrading MucA. We have identified three candidates for these additional proteases in the bacterium's genome. We are beginning experiments to determine if one of our candidates actually does play a role in regulating alginate production. These experiments will enable us to understand how the production of alginate in induced in virulent strains by the degradation of MucA.