DII LABELING OF GUSTATORY AFFERENTS TO THE ZEBRAFISH VAGAL LOBE

该子项目是利用NIH/NCRR资助的中心赠款提供的资源的许多研究子项目之一。子项目和研究者（PI）可能从另一个NIH来源获得了主要资金，因此可以在其他CRISP条目中表示。所列机构为中心机构，不一定为研究者机构。迷走神经叶是鱼类后脑背面的一个扩大部分，具有发达的味觉，特别是在咽部。鲤鱼科的一些成员，主要是鲤鱼和金鱼，已经对迷走神经叶组织进行了广泛的研究，但对其他相关物种迷走神经叶的内在组织知之甚少。在这个建议所涵盖的时间内，我将标记的斑马鱼咽传入与死后荧光神经元示踪剂，二碘。斑马鱼是一个很有吸引力的模型，因为它们代表了鲤科鱼类中一个知之甚少、发展较晚的成员。描述斑马鱼迷走神经叶内神经回路的组织结构是比较简单和复杂味觉系统的必要的第一步。我将从我的同事那里获得15-20个固定的斑马鱼标本，他们对嗅觉系统进行生理学研究。我们将用DiI标记舌咽（IX）和迷走（X）神经的选定分支，并等待7-14天以使标记物在细胞内扩散。大脑将在50岁时取出并切片我在荧光灯下分析。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The vagal lobe is an enlargement of the dorsal surface of the hindbrain of fishes that have a well-developed sense of taste, particularly in the pharynx. Selected members of the cyprinid family, mostly carps and goldfish, have been studied extensively for vagal lobe organization, but very little is known about the intrinsic organization of the vagal lobe of other related species. During the time covered by this proposal, I would label the pharyngeal afferents of zebrafish with a post-mortem fluorescent neuronal tracer, DiI. Zebrafish are an appealing model because they represent a poorly understood, less developed member of the cyprinid family. Characterizing the organization of the neuronal circuitry within the zebrafish vagal lobe is a necessary first step in comparing simple and complex gustatory systems. I will acquire 15-20 fixed zebrafish specimens from associates of mine who conduct physiological studies on the olfactory system. We will label selected branches of the glossopharyngeal (IX) and vagal (X) nerves with DiI and wait 7-14 days for intracellular diffusion of the label. The brains will be removed and sectioned at 50 ?m to analyze under epifluorescence.