VERMONT IMMUNOBIOL/INFECTIOUS DIS CTR: MIICROARRAY & BIOINFORMATIC ANALYSIS CORE

这个子项目是利用由NIH/NCRR资助的中心拨款提供的资源的许多研究子项目之一。子项目和调查员(PI)可能从另一个NIH来源获得了主要资金，因此可能会出现在其他CRISE条目中。列出的机构是针对中心的，而不一定是针对调查员的机构。微阵列公司的核心设施采用Affymetrix基因芯片2500系统，该系统包括杂交站640、流体站400和扫描仪2500。安捷伦生物分析仪2100也可用于RNA分析。基因芯片表达分析探针阵列从Affymetrix购买。微阵列核心还配备了各种工具，用于数据分析、联网和数据库管理。使用微阵列套件、DMT、Micro DB、GeneSpring和gene Sifter软件包对数据进行分析。微阵列核心提供RNA表达分析服务，包括RNA的质量测试、与基因芯片探针阵列的杂交、图像处理、初级数据分析、数据传输以及生物信息学工具的使用，这些工具由生物信息学核心管理。可使用各种软件包为目标准备和数据分析提供用户支持。生物信息学核心设施由一名Unix系统管理员和两名程序员提供支持。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The Microarray core facility utilizes the Affymetrix GeneChip 2500 system that includes Hybridization Station 640, Fluidics Station 400 and Scanner 2500. An Agilant bioanalyzer 2100 is also available for RNA analysis. GeneChip expression analysis probe arrays are purchased from Affymetrix. The microarray core is also equipped with a variety of tools for data analysis, networking and database management. Data are analyzed using the Microarray Suite, DMT, Micro DB, GeneSpring and Gene Sifter software packages. The Microarray Core provides RNA expression-profiling services including quality testing of RNA, hybridization to GeneChip probe arrays, image processing, primary data analysis, data delivery, and access to Biomformatics tools, which are managed by the Bioinformatics Core. User support is available for target preparation and data analysis using various software packages. The Bioinformatics core facility provides support with a Unix system administrator and two programmers.