LSUHSC COBRE: CORE 1: IMMUNOLOGY & CELL ANALYSIS

该子项目是利用NIH/NCRR资助的中心赠款提供的资源的许多研究子项目之一。子项目和研究者（PI）可能从另一个NIH来源获得主要资金，因此可以在其他CRISP条目中表示。所列机构为中心机构，不一定为研究者机构。免疫学和细胞分析核心（ICAC）是COBRE中提出的科学项目发展的核心。它将为PJI提供设备和技术支持。免疫学和细胞分析核心将具有可用的细胞技术，包括从外周血和组织中分离细胞亚群，通过流式细胞术和组织学对细胞进行表型表征，以及对细胞功能进行表征，包括增殖、细胞周期分析、细胞因子产生和细胞毒性。此外，ICAC还将提供研究细胞信号传导的能力，如酪氨酸激酶磷酸化、钙流和核转录因子的活化和易位。此外，分子免疫学技术包括北方印迹、PCR、RT-PCR、真实的时间PCR、Run-On和Run-Off实验、转染、干扰RNA（RNAi）、RNA酶保护测定（RPA）和其它目前在ICAC实验室中进行。廉政公署亦会协助收集、冷冻及储存活细胞及血清样本，以供日后研究之用。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The Immunology and Cell Analysis Core (ICAC) is central to the development of the scientific projects proposed in this COBRE. It will provide access to equipment and technical support to the PJI. The Immunology and Cell Analysis Core will have available cellular techniques including separation of cell subpopulations from peripheral blood and tissues, phenotypic characterization of the cells by flow cytometry and histology and characterization of cell functions including proliferation, cell cycle analysis, cytokine production and cytotoxicity. In addition the ICAC will also provide the capacity to study cell signaling such as tyrosine kinase phosphorylation, calcium flux and activation and translocation of nuclear transcription factors. In addition molecular immunology techniques including Northern blots, PCR, RT-PCR, real time PCR, Run- On and Run-Off experiments, transfection, interfering RNA (RNAi), RNAse protection assay (RPA) and others are currently done in the ICAC laboratory. The ICAC will also provide support in the collection, freezing and storage of viable cells and serum samples for future studies.