COBRE: U OF KANSAS MEDICAL CTR: CORE B: MOLECULAR BIOLOGY CORE

该子项目是利用NIH/NCRR资助的中心赠款提供的资源的许多研究子项目之一。子项目和研究者（PI）可能从另一个NIH来源获得主要资金，因此可以在其他CRISP条目中表示。所列机构为中心机构，不一定为研究者机构。分子生物学核心将为所有COBRE科学家提供技术支持，最先进的设备和必要的分子生物学试剂，以便以具有成本效益的方式进行需要分子生物学方法的实验。核心将提供中央DNA测序设施，实时PCR服务以量化mRNA表达，高通量PCR和基因分型服务，以及用于共享的载体，质粒和细菌的中央库。核心将为必要设备提供一个中心位置，包括ABI Prism 3100基因分析仪（当前申请中要求），实时PCR仪（到位），Biomek FX检测工作站（液体分配器，到位）和5个热循环仪（在适当的位置），以及其他常用的分子生物学设备，例如具有密度测定分析能力的成像系统，细菌培养器和超浓缩器。核心将配备经验丰富的分子生物学家，他们不仅在使用这种设备方面有实践经验，而且在管理核心设施方面有良好的记录。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The Molecular Biology Core will provide all COBRE scientists with technical support, state-of-the-art equipment, and essential molecular biology reagents, so that experiments which require a molecular biology approach can be carried out in a cost-effective way. The Core will provide a central DNA-sequencing facility, real-time PCR services to quantify mRNA expression, high-throughput PCR and genotyping services, and a central bank of vectors, plasmids, and bacteria for sharing. The Core will provide a central location for essential equipment, including an ABI Prism 3100 Genetic Analyzer (requested in the current application), a real-time PCR machine (in place), a Biomek FX Assay Work Station (liquid dispenser, in place), and 5 thermocyclers (in place), and other commonly used molecular biology equipment, such as an imaging system with the capability for densitometry analysis, bacteria incubators, and ultracentrifuges. The Core w II be staffed with experienced molecular biologists, who not only have hands-on experience in using this equipment, but also a proven track record in managing a core facility.