TMEM103 in Eosinophil Development

描述（由申请人提供）：嗜酸性粒细胞与许多过敏性疾病有关，包括哮喘、特应性皮炎、药物反应和慢性荨麻疹。无数的嗜酸性粒细胞相关疾病促使我们研究造血干细胞中嗜酸性粒细胞的发展，试图了解促使骨髓中嗜酸性粒细胞产生的信号。发育中的嗜酸性粒细胞的基因谱分析表明，一种新的假设蛋白以mRNA的形式表达。tmem103mrna在IL-5刺激的CD34+细胞、骨髓和成熟嗜酸性粒细胞中表达。转录本TMEM103已被证明与核糖体相关，该蛋白已通过western blot分析在发展中的嗜酸性粒细胞中被鉴定出来。本基金拟进一步验证TMEM103的存在，方法是培养该蛋白的单克隆抗体，然后对该蛋白进行纯化和测序。此外，TMEM103的功能将通过RNA沉默以及形态学和转录谱变化的研究来研究。公共卫生相关性：嗜酸性粒细胞是与哮喘和过敏性疾病相关的白细胞。本项目探讨了TMEM103（一种新型蛋白）在嗜酸性粒细胞中的作用。

DESCRIPTION (provided by applicant): Eosinophils are associated with many allergic diseases including asthma, atopic dermatitis, drug reactions and chronic urticaria. The myriad of eosinophil related diseases has led us to investigate the development of eosinophils from hematopoietic stem cells in an attempt to understand the signals prompting eosinophil production in the bone marrow. Gene profiling of developing eosinophils has shown that a novel hypothetical protein is expressed as mRNA. TMEM103 mRNA is expressed in IL-5 stimulated CD34+ cells, bone marrow and in mature eosinophils. The transcript, TMEM103, has been shown to be associated with ribosomes and the protein has been identified by western blot analysis in developing eosinophils. This grant proposes to further verify the existence of TMEM103 by raising monoclonal antibodies to the protein, then purifying and sequencing the protein. Further, the function of TMEM103 will be investigated by RNA silencing followed by studies investigating morphological and transcription profile changes. PUBLIC HEALTH RELEVANCE: Eosinophils are leukocytes associated with asthma and allergic diseases. This project explores the role of TMEM103, a novel protein, in eosinophils.