Improving Biomaterials from a Cellular Point of View

描述（由申请人提供）：在本提案中，我们将研究一种潜在的骨愈合新方法。众所周知，植入物材料已用于支持组织修复或再生多年。现在认为，这种植入物也可能通过活组织和细胞诱导自然骨愈合反应。具体而言，生物活性玻璃已经显示出通过释放生物活性玻璃离子来诱导成骨细胞对骨愈合的反应的能力。本研究的目的是探索生物活性玻璃离子在骨愈合和骨再生中发挥积极作用的新观点。 为了研究这些离子对骨愈合的影响，我们将从细胞内和细胞外的角度研究这些玻璃如何与细胞相互作用。首先，人们认为，生物活性玻璃离子，这是由玻璃在浸泡在生理液体中释放，可诱导成骨细胞的各种反应。其次，这些生物活性玻璃离子（即硅和钙）可以组合控制成骨细胞功能，从而加速或增强骨再生。最后，我们研究了如何生物活性玻璃离子释放可以控制使用编程的生物活性玻璃。用于确定生物活性玻璃离子对基因表达和细胞外活性的影响的技术包括基因微阵列、真实的时间聚合物链反应、免疫测定和免疫组织化学。 为了编程生物活性玻璃，化学气相沉积用于构建纳米层和微米层复合玻璃，其将目标离子浓度递送到细胞以增强成骨细胞功能。这项工作的目的和目标符合NIH的使命，即通过提高我们对细胞-生物材料相互作用的理解和探索制造“智能”生物材料的新方法来改进生物材料。这里提出的工作研究生物医学设备细胞相互作用的骨愈合。具体而言，我们希望通过在生理液体中控制生物材料降解来控制和改善骨愈合。拟议的工作旨在开发用于基因相关疗法的自我调节材料。因此，提高我们对组织-生物医学器械相互作用的理解可以导致改善组织愈合并减轻患者因衰弱状况和疾病而遭受的痛苦的器械。

DESCRIPTION (provided by applicant): In this proposal, we will investigate a potential new way of bone healing. It is widely known that implant materials have been used for many years to support tissue repair or regeneration. It is now thought that such implants may also induce natural bone healing responses by living tissues and cells. Specifically, bioactive glasses have shown this ability to induce osteoblast response for bone healing by releasing bioactive glass ions. The purpose of this proposed work is to explore this novel idea that bioactive glass ions play an active role in bone healing and bone regeneration. To investigate the influence of these ions on bone healing, we will examine how these glasses interact with cells from an intracellular and extracellular perspective. First, it is believed that bioactive glass ions, which are release by glasses during immersion in physiological fluid, may induce various responses by osteoblasts. Second, these bioactive glass ions (namely silicon and calcium) may combinatorially control osteoblast function such that bone regeneration is hastened or enhanced. Finally, we investigate how bioactive glasses ion release can be controlled using a programmed bioactive glass. The techniques used to determine the influence of bioactive glass ions on gene expression and extracellular activity includes gene microarrays, real time polymer chain reaction, immunoassays, and immunohistochemistry. To program bioactive glasses, chemical vapor deposition is used to build nanolayered and microlayered composite glasses that deliver target ion concentrations to cells for enhanced osteoblast function. The aims and goals of this proposed work fit into the mission of NIH, that is, improving biomaterials by improving our understanding of cell-biomaterial interactions and exploring new methods in fabricating "smart" biomaterials. The work proposed here investigates biomedical device-cell interactions for bone healing. Specifically, we wish to control and improve bone healing through controlled biomaterial degradation in physiological fluid. The proposed work is aimed at developing materials that are self-regulating for gene-related therapies. Therefore, improving our understanding of tissue-biomedical device interactions can lead to devices that improve tissue healing and ease patient suffering owed to debilitating conditions and diseased.