HORMONE ASSAY CORE

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 激素检测核心实验室是CRC的第一个组成部分，将在资金后开发。 激素检测核心实验室的目标是通过开发和提供目前研究人员无法获得的激素测量方法来扩大查尔斯德鲁大学的激素检测能力。 激素检测核心实验室在改进现有检测方法以满足研究人员的特定需求以及开发新检测方法方面取得了相当大的成功。以下是目前可用的激素检测列表： 1.血清LH：双位点定向荧光免疫测定（FIA）。 2.血清FSH：双位点定向荧光免疫测定（FIA）。 3.特伦斯：睾酮测定使用碘化示踪剂，并在免疫测定前使用己烷：乙酸乙酯（2：3）混合物提取血清样品。该实验室已经发表了一种高度灵敏的睾酮测定方法的开发，该方法已被优化用于测量女性的低睾酮水平。 4.游离睾酮：游离睾酮水平通过直接平衡透析程序测量。该实验室已经发表了用于测量女性低游离睾酮水平的灵敏测定的开发和验证，以及在怀孕女性中生成规范性数据。 5.二氢睾酮：提取血清样品，并通过硅藻土柱进行色谱分析。通过RIA测量来自硅藻土级分的滤液中的DHT浓度。 6.性激素生成球蛋白（SHBG）：血清SHBG结合能力通过使用示踪量的[3 H-DHT]和系列稀释的DHT标准品来测量。 7.胰岛素：胰岛素测定使用来自Wellcome的等同于第一个1 RP GG/304的标准品、来自ICN的抗猪抗体和来自Lilly的猪胰岛素进行碘化。 8. C肽：C肽测定使用Eli Lilly Company提供的抗血清、标准品和碘化材料以及乙醇分离技术。雄烯二酮、脱氢表雄酮、雌酮、雌二醇、肌肉生长抑制素、炎症标志物、C反应蛋白和IL-6。 9.自获得初始资金以来，HAC实验室提供了最先进的服务，并在开发新的检测方法方面具有高度创新性。HAC实验室鼓励分子生物学家和临床研究人员之间的合作。HAC实验室有着杰出的成就记录;它支持了大量具有高影响力的同行评审出版物和新的RO 1资助申请。 注：核心不进行任何自己的研究。 它为CRC和IRB批准的研究提供激素测量。 因此，它使用与CRC伞式赠款相同的IRB批准。 核心研究中心未入组患者。 这是一个纯粹的激素测量实验室。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The Hormone Assay Core Lab was the first component of the CRC to be developed following funding. The objective of the Hormone Assay Core Lab is to expand the hormone assay capabilities at Charles Drew University by developing and making available hormone measurement methods not currently available to the investigators. The Hormone Assay Core Laboratory has had considerable success in refining existing assays to meet specific needs of investigators, and in developing new assays. Below is a list of hormone assays that are currently available: 1. Serum LH: A 2-site directed fluoroimmunometric assay (FIA). 2. Serum FSH: A 2-site directed fluoroimmunometric assay (FIA). 3. Testosterone: The assay for testosterone uses an iodinated tracer and extraction of serum samples using anexane: ethyl acetate (2:3) mixture, prior to immunoassay. The laboratory has published the development of a highly sensitive testosterone assay that has been optimized for the measurement of low testosterone levels in women. 4. Free Testosterone: Free testosterone levels are measured by a direct equilibrium dialysis procedure. The laboratory has published the development and validation of the sensitive assay for the measurement of low free testosterone levels in women, and the generation of normative data in menstruating women. 5. Dihydrotestosterone: Serum samples are extracted and chromatographed through a Celite column. DHT concentrations are measured in the eluate from the Celite fractions by RIA. 6. Sex-Hormone Building Globulin (SHBG): Serum SHBG binding capacity is measured by using tracer amounts of [3H-DHT] and serial dilutions of DHT standards. 7. Insulin: Insulin assay uses a standard from Wellcome equated to 1st 1RP GG/304, antiporcine antibody from ICN and porcine insulin from Lilly for iodination. 8. C-Peptide: C-Peptide assay uses antisera, standards and iodination material supplied through the Eli Lilly Company and an ethanol separation technique. Androstenedione, DHEA, Estrone, estradiol, myostatin, inflammatory markers, C-reactive protein and IL-6. 9. Since its initial funding, the HAC Lab has provided state-of-the-art service, and has been highly innovative in developing new assays. The HAC Lab has encouraged collaborations between molecular biologists and clinical investigators. The HAC Lab has had an outstanding record of accomplishments; it has supported a large number of high-impact, peer-reviewed publications, and new RO1 grant applications. Note: The Core does not conduct any studies of it''s own. It provides hormone measurements to studies that are CRC and IRB approved. Therefore, it uses the same IRB approval as the umbrella CRC grant does. There is no patient enrollment by the Core. It is purely a hormone measurement laboratory.