HORMONE ASSAY CORE

本子项目是利用由NIH/NCRR资助的中心赠款提供的资源的众多研究子项目之一。子项目和研究者（PI）可能已经从另一个NIH来源获得了主要资金，因此可以在其他CRISP条目中表示。列出的机构是中心的，不一定是研究者的机构。激素测定核心实验室是获得资助后开发的CRC的第一个组成部分。激素检测核心实验室的目标是通过开发和提供目前研究人员无法获得的激素测量方法，扩大查尔斯德鲁大学的激素检测能力。激素检测核心实验室在改进现有检测方法以满足研究人员的特定需求和开发新检测方法方面取得了相当大的成功。以下是目前可用的激素检测方法：血清LH：一种2位点定向荧光免疫测定（FIA）。2. 血清卵泡刺激素：一种2位点定向荧光免疫测定法（FIA）。3. 睾酮：睾酮的测定使用碘化示踪剂，在免疫测定之前，使用乙酸乙酯（2:3）混合物提取血清样本。该实验室发表了一项高灵敏度睾酮测定法的开发成果，该方法已优化用于测量女性低睾酮水平。4. 游离睾酮：游离睾酮水平通过直接平衡透析程序测量。该实验室发表了开发和验证用于测量妇女游离睾酮水平低的敏感分析方法，并产生经期妇女的规范数据。5. 二氢睾酮：提取血清样品并通过Celite色谱柱进行色谱分析。二氢睾酮浓度通过RIA测定Celite馏分洗脱液。6. 性激素构建球蛋白（SHBG）：通过使用[3H-DHT]示踪剂量和DHT标准品的连续稀释来测量血清SHBG的结合能力。7. 胰岛素：胰岛素测定采用惠康公司的1RP GG/304标准品，ICN公司的抗猪抗体和礼来公司的猪胰岛素进行碘化。8. C-Peptide: C-Peptide检测使用Eli Lilly公司提供的抗血清、标准品和碘化材料以及乙醇分离技术。雄烯二酮、脱氢表雄酮、雌酮、雌二醇、肌肉生长抑制素、炎症标志物、c反应蛋白和IL-6。9. 自获得初始资金以来，HAC实验室提供了最先进的服务，并在开发新的检测方法方面具有高度创新性。HAC实验室鼓励分子生物学家和临床研究者之间的合作。HAC实验室取得了杰出的成就；它支持了大量高影响力、同行评审的出版物和新的RO1拨款申请。注：核心不进行任何自己的研究。它为CRC和IRB批准的研究提供激素测量。因此，它使用与总括性CRC授权相同的IRB批准。Core没有患者登记。它纯粹是一个激素测量实验室。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The Hormone Assay Core Lab was the first component of the CRC to be developed following funding. The objective of the Hormone Assay Core Lab is to expand the hormone assay capabilities at Charles Drew University by developing and making available hormone measurement methods not currently available to the investigators. The Hormone Assay Core Laboratory has had considerable success in refining existing assays to meet specific needs of investigators, and in developing new assays. Below is a list of hormone assays that are currently available: 1. Serum LH: A 2-site directed fluoroimmunometric assay (FIA). 2. Serum FSH: A 2-site directed fluoroimmunometric assay (FIA). 3. Testosterone: The assay for testosterone uses an iodinated tracer and extraction of serum samples using anexane: ethyl acetate (2:3) mixture, prior to immunoassay. The laboratory has published the development of a highly sensitive testosterone assay that has been optimized for the measurement of low testosterone levels in women. 4. Free Testosterone: Free testosterone levels are measured by a direct equilibrium dialysis procedure. The laboratory has published the development and validation of the sensitive assay for the measurement of low free testosterone levels in women, and the generation of normative data in menstruating women. 5. Dihydrotestosterone: Serum samples are extracted and chromatographed through a Celite column. DHT concentrations are measured in the eluate from the Celite fractions by RIA. 6. Sex-Hormone Building Globulin (SHBG): Serum SHBG binding capacity is measured by using tracer amounts of [3H-DHT] and serial dilutions of DHT standards. 7. Insulin: Insulin assay uses a standard from Wellcome equated to 1st 1RP GG/304, antiporcine antibody from ICN and porcine insulin from Lilly for iodination. 8. C-Peptide: C-Peptide assay uses antisera, standards and iodination material supplied through the Eli Lilly Company and an ethanol separation technique. Androstenedione, DHEA, Estrone, estradiol, myostatin, inflammatory markers, C-reactive protein and IL-6. 9. Since its initial funding, the HAC Lab has provided state-of-the-art service, and has been highly innovative in developing new assays. The HAC Lab has encouraged collaborations between molecular biologists and clinical investigators. The HAC Lab has had an outstanding record of accomplishments; it has supported a large number of high-impact, peer-reviewed publications, and new RO1 grant applications. Note: The Core does not conduct any studies of it''s own. It provides hormone measurements to studies that are CRC and IRB approved. Therefore, it uses the same IRB approval as the umbrella CRC grant does. There is no patient enrollment by the Core. It is purely a hormone measurement laboratory.