In vivo Imaging of Hypoxic-Ischemic Encephalopathy in Neonatal Mice

描述(由申请人提供)：缺氧缺血性脑病(HIE)损害婴儿的早产儿白质和灰质，并导致显著的死亡率和发病率。为了找到满意的治疗方法，人们建立了HIE的动物模型，并广泛应用。在这个项目中，将开发新的磁共振成像技术来研究新生小鼠HIE模型脑损伤的时间演变。重点将放在开发用于监测新生动物损伤的快速成像技术，以及基于模板的新技术来评估脑损伤的程度和进展，特别是在发育中的白质。在目标1中，我们将开发一种活体新生小鼠成像系统。在目标2中，我们将开发基于模板的技术来评估灰质和白质损伤。在目标3中，我们将评估由HIE引起的特定部位的损伤。我们将使用这些技术来表征缺氧缺血性脑病新生小鼠灰质和白质损伤的时间演变，并将结果与病理相关联。这一结果将为HIE的研究提供关键信息，该技术也可应用于其他动物模型。总之，这项提议将建立一个模型成像系统，用于探索缺氧缺血性脑病的新治疗方法。与公共卫生相关的缺氧缺血性脑病(HIE)导致婴儿大量死亡和发病。在本项目中，将开发新的磁共振成像技术来研究新生小鼠HIE模型中脑损伤的时间演变，以探索治疗缺氧缺血性脑病的新方法。

DESCRIPTION (provided by applicant): Hypoxic-ischemic encephalopathy (HIE) damages premature white matter and gray matter in infants and causes significant mortality and morbidity. To find satisfactory treatments, animal models of HIE have been established and widely used. In this project, novel magnetic resonance imaging techniques will be developed to examine temporal evolution of brain injury in a neonatal mouse model of HIE. Focus will be placed on the development of fast imaging techniques for monitoring injuries in neonatal animals, and new template based techniques to assess the extent and progression of brain injury, especially in the developing white matter. In aim #1, we will develop an in vivo neonatal mouse imaging system. In aim #2, we will develop template based techniques to assess gray and white matter injuries. In aim #3, we will evaluate tract specific injuries caused by HIE. We will use these techniques to characterize the temporal evolution of gray and white matter injuries in neonatal mice with HIE and correlate the results with pathology. The results will provide key information for study of HIE, and the techniques can be applied to other animal models. In summary, this proposal will establish a model imaging system for exploration of novel treatments of hypoxic-ischemic encephalopathy. PUBLIC HEALTH RELEVANCE Hypoxic-ischemic encephalopathy (HIE) causes significant mortality and morbidity in infants. In this project, novel magnetic resonance imaging techniques will be developed to examine temporal evolution of brain injury in a neonatal mouse model of HIE, for exploration of novel treatments of hypoxic-ischemic encephalopathy.