Mapping gene expression in the developing mouse brain

绘制小鼠大脑发育中的基因表达图谱

• 批准号: 8516121
• 负责人: JIANGYANG ZHANG
• 金额: $ 33.93万
• 依托单位: JOHNS HOPKINS UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-09-01 至 2015-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8516121
• 关键词: Anatomy; Area; Atlases; Base of the Brain; Brain; Brain region; Cell Lineage; Cells; Computational Technique; Computer software; Data; Databases; Development; Disease; Embryo; Ensure; Exhibits; Foundations; Gene Expression; Gene Expression Profile; Genes; Genetic; Genetic Code; Goals; Grant; Hour; Human; Image; Imagery; Imaging Techniques; In Situ Hybridization; Laboratory mice; Lead; Magnetic Resonance; Magnetic Resonance Imaging; Maps; Measurement; Morus; Mus; Neonatal; Neuraxis; Neuroanatomy; Pattern; Phenotype; Play; Population; Process; Public Domains; Reference Values; Research; Resolution; Resources; Role; Series; Specificity; Staging; Structure; System; Techniques; Tissue Differentiation; Tissues; base; cell motility; cell type; mind control; postnatal; public health relevance; tool

DESCRIPTION (provided by applicant): Gene expression in the brain is important for understanding the genetic control of brain development. Because the laboratory mouse shares many genes and disease loci with human, its gene expression patterns are important for research in this area. With modern high throughput techniques, large scale gene expression data in the mouse brain are becoming available, and several mouse gene expression databases are being established. To integrate and analyze gene expression data with neuroanatomy in the embryonic and neonatal mouse brains, it is necessary to create three dimensional reference atlases of the developing mouse brain and tools that can map gene expression data into the atlas and perform analysis and visualization. The proposed study will establish a series of three dimensional reference atlases of the developing mouse brain based on high spatial resolution and rich tissue contrast provided by modern magnetic resonance imaging techniques. The atlases will contains structural annotation/segmentation, and more importantly, information on morphological changes during development obtained using advanced computational techniques. The proposed study will also develop tools that mapping gene expression data to the reference atlases. A selected group of gene expressions will be mapped, validated, and studied. The mapped gene expression data will also help us to define more detailed structures in the developing mouse brain atlases. PUBLIC HEALTH RELEVANCE: In this project, three dimensional atlases of the developing mouse brain will be developed to serve as the reference for mapping high resolution gene expression data. The atlases will consist of high resolution, rich contrast, and group average data that cover the key embryonic to neonatal stages during brain development. Techniques and software for mapping gene expression data to the atlases will also be developed, validated and used to investigate the relationship between gene expression and anatomical phenotypes as well as further enrich the structural annotation in the atlases.

描述（由申请人提供）：大脑中的基因表达对于理解大脑发育的遗传控制是重要的。由于实验小鼠与人类有许多共同的基因和疾病位点，其基因表达模式对该领域的研究具有重要意义。随着现代高通量技术的发展，小鼠脑中大规模的基因表达数据变得可用，并且正在建立几个小鼠基因表达数据库。为了整合和分析胚胎和新生小鼠脑中的基因表达数据与神经解剖学，有必要创建发育中小鼠脑的三维参考图谱以及可以将基因表达数据映射到图谱中并执行分析和可视化的工具。 该研究将基于现代磁共振成像技术提供的高空间分辨率和丰富的组织对比度，建立一系列发育中小鼠大脑的三维参考图谱。地图集将包含结构注释/分割，更重要的是，使用先进的计算技术获得的发育期间形态变化的信息。拟议的研究还将开发将基因表达数据映射到参考图谱的工具。一组选定的基因表达将被映射，验证和研究。绘制的基因表达数据也将帮助我们在发育中的小鼠大脑图谱中定义更详细的结构。 公共卫生关系：在这个项目中，将开发小鼠大脑发育的三维图谱，作为绘制高分辨率基因表达数据的参考。这些地图集将包括高分辨率、丰富的对比度和组平均数据，涵盖大脑发育过程中从胚胎到新生儿的关键阶段。还将开发、验证将基因表达数据映射到图谱的技术和软件，并用于研究基因表达与解剖表型之间的关系，以及进一步丰富图谱中的结构注释。