Sequencing the 'gene space' of potato chromosome IV comparative analysis with tomato and development of a gene-based mapping platform

这项提议将允许英国参与一项国际合作，通过对四号染色体进行测序，确定马铃薯基因组中“基因丰富”区域的DNA序列。其他染色体正在由其他国家的研究小组进行测序。马铃薯是世界上第四大粮食作物，是茄科最重要的成员，茄科还包括番茄、辣椒、茄子和许多与医学有关的植物。马铃薯的基因组与番茄非常相似，由于英国也在对番茄的第四染色体进行测序，我们将能够对这两种作物进行非常详细的比较序列分析。此外，通过研究基因和基因组序列数据，我们将能够创建一个基于不同品种之间微小DNA变异的“遗传标记”系统。这将使我们能够很容易地检查大量的基因，并看到它们在具有不同特征的品种之间如何变化。这将对培育马铃薯作物的努力非常有用，这些作物可以以更环保和可持续的方式种植。所产生的所有数据将以用户方便的格式放在公共领域，并确定假定的基因及其功能。该项目还将用于每年将从事茄科植物研究的英国研究界聚集在一起，以交流他们的研究成果。

This proposal will allow the UK to participate in an international collaboration to determine the DNA sequence of the 'gene-rich' regions of the potato genome, by sequencing chromosome IV. The other chromosomes are being sequenced by groups in other countries. Potato, the world's fourth most important food crop, is the most important member of the Solanaceae, which also includes tomato, pepper, aubergine and many medically relevant plants. The potato genome is very similar to tomato and, as the UK is also sequencing tomato chromosome IV, we will be able to perform a highly detailed comparative sequence analysis of these two crop species. Moreover, by studying gene and genome sequence data we will be able to create a 'genetic marker' system, based on small DNA variations between different varieties. This will allow us to readily examine a large number of genes and to see how they vary between varieties with different characteristics. This will be extremely useful for efforts to breed potato crops that can be grown in a more environmentally friendly and sustainable manner. All of the data generated will be placed in the public domain in a user-ready format with the identification of putative genes and their function. The project will also be used to bring the UK research community working on Solanaceous plants together each year for the purposes of communicating the results of their research.