LOCATING DISULFIDE BONDS IN CALCIUM-BINDING PROTEIN

本子项目是利用由NIH/NCRR资助的中心赠款提供的资源的众多研究子项目之一。子项目和研究者（PI）可能已经从另一个NIH来源获得了主要资金，因此可以在其他CRISP条目中表示。列出的机构是中心的，不一定是研究者的机构。钙结合蛋白被认为是组织浆体**中的毒力因子，它被排泄到巨噬细胞宿主中，导致人类肺部真菌感染。核磁共振结构已基本确定，但C2对称蛋白的二聚化引起不确定性。此外，蛋白质有3个二硫键，这些不是由核磁共振确定的。我们正在使用蛋白质水解和同位素丰度来确定二硫键模式，这将使核磁共振数据在结构确定方面受到约束。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Calcium Binding Protein is believed to be the virulence factor in Histoplasma ** and it is excreted into the macrophage host leading to fungal infection of the lung in humans. The NMR structure has been nearly determined, but the dimerization of the C2 symmetry protein causes uncertainty. Additionally, the protein has 3 disulfide bonds and these are not determined by the NMR. We are using proteolysis and isotope abundance to determine the disulfide bonding pattern and this will enable the NMR data to be constrained for structure determination.