LEUKOTOXIN DIOL FROM CORN COB ANIMAL BEDDING STIMULATES BREAST CANCER CELLS

本子项目是利用由NIH/NCRR资助的中心赠款提供的资源的众多研究子项目之一。子项目和研究者（PI）可能已经从另一个NIH来源获得了主要资金，因此可以在其他CRISP条目中表示。列出的机构是中心的，不一定是研究者的机构。玉米芯含有多种促有丝分裂剂，可刺激体内和体外的乳腺癌和前列腺癌细胞增殖，并破坏男性和女性的生殖功能和周期。用高效液相色谱法对玉米芯层理提取物进行纯化，发现含有9,10 -二羟基-12-十八烯酸（ltx -二醇）的C8反相柱洗脱有丝分裂活性的主峰。这两种位置异构体（LTX-diols）的混合物在13-26 μ m的浓度范围内刺激MCF-7人乳腺癌细胞的体外增殖。因此，来自外源性或内源性来源或途径的ltx -二醇可能促进或增强恶性细胞增殖，我们怀疑是内分泌的。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Ground corncob contains a number of mitogenic agents that stimulate breast and prostate cancer cell proliferation in vitro and in vivo and also disrupt male and female reproductive function and cyclicity. Purification of ground corncob bedding extracts by HPLC revealed a major peak of mitogentic activity eluting from a C8 reversed phase column that contained 9, 10-dihydroxy-12-octadecenoicacid (LTX-diol), a well-known leukotoxin-diol. This mixture of the two positional isomers (LTX-diols) stimulated the proliferation of MCF-7 human breast cancer cells in vitro at concentrations ranging from 13-26 uM. Thus, LTX-diol derived from exogenous or endogenous sources or pathways may promote or enhance malignant cell proliferation that we suspect are endocrine.