WHOLE BODY LEUCINE KINETICS DURING ACUTE INFECTION DECREASED BY MALNUTRITION

这个子项目是利用由NIH/NCRR资助的中心拨款提供的资源的许多研究子项目之一。子项目和调查员(PI)可能从另一个NIH来源获得了主要资金，因此可能会出现在其他CRISE条目中。列出的机构是针对中心的，而不一定是针对调查员的机构。这项研究比较了三组马拉维儿童的亮氨酸动力学和急性期蛋白和细胞因子浓度，这些儿童接受等能量、等氮饮食：有消瘦症状的儿童(n=25)和没有感染的儿童(n=17)，以及营养良好的感染儿童(n=13)。被测试的假设是，软体急性感染儿童的全身亮氨酸动力学将低于营养良好的急性感染儿童，但高于软体未感染儿童。治疗24小时后，采用标准的13C-亮氨酸稳定同位素示踪技术对儿童进行研究。营养良好的急性感染儿童的亮氨酸代谢速率高于消瘦的急性感染儿童；非氧化性亮氨酸处理组为153+/-31比118+/-43[mU]·L·h-1，来自全身蛋白分解的亮氨酸为196+/-34比121+/-47，亮氨酸氧化为85+/-31比45+/-13(p&lt；均为0.01)。患有和不患有急性感染的软体病儿的亮氨酸动力学速率相似。营养良好的急性感染儿童在最初的24小时内六种急性时相蛋白中的五种增加了他们的血清浓度，而患有马拉松症感染的儿童没有任何增加。血清IL-6浓度在营养良好和软体病合并感染的儿童中升高。这些数据表明，对急性感染的急性期蛋白质动力学反应的细胞因子刺激存在于消瘦儿童中，但营养不良使急性期蛋白质代谢反应变得迟钝。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. This study compared leucine kinetics and acute-phase protein and cytokine concentrations in three groups of Malawian children who were fed an isoenergetic, isonitrogenous diet: children with marasmus with (n = 25) and without (n = 17) infection and well-nourished children with infection (n = 13). The hypotheses tested were that whole-body leucine kinetics will be less in marasmic acutely infected children than in well-nourished acutely infected children but greater than in marasmic uninfected children. Children were studied after 24 h of therapy using standard 13C-leucine stable isotope tracer techniques. Well-nourished children with acute infection had greater leucine kinetic rates than did marasmic children with acute infection; nonoxidative leucine disposal was 153 +/- 31 versus 118 +/- 43 [mu]mol leucine kg-1 h-1, leucine derived from whole-body proteolysis was 196 +/- 34 versus 121 +/- 47, and leucine oxidation was 85 +/- 31 versus 45 +/- 13 (p < 0.01 for all comparisons). Leucine kinetic rates were similar in marasmic children with and without acute infection. Well-nourished children with acute infection increased their serum concentration of five of six acute-phase proteins during the first 24 h, whereas marasmic children with infection did not have any increases. The serum concentrations of IL-6 were elevated in well-nourished and marasmic children with infection. These data suggest that the cytokine stimulus for the acute-phase protein kinetic response to acute infection is present in marasmic children but that the acute-phase protein metabolic response is blunted by malnutrition.