Typhoon Trio Imager

描述（由申请人提供）：本建议书申请资金购买台风三人成像仪工作站。该成像工作站允许高灵敏度，定量数据成像分析，通过六种不同的扫描模式-四色荧光，磷光成像和化学发光检测在一个单一的高通量系统。目前在科罗拉多州立大学的主校区没有其他类似的设备，用户组目前依赖于位于该大学另一个学院MRB大楼5分钟路程的过时且过度使用的风暴磷光仪。该科将主要为微生物、免疫及病理学系的65名教职员提供影像服务。该科在2005年的校外研究经费超过3,800万元。由于影像工作站所支援的技术在分子生物学领域已变得司空见惯，该单位将使整个部门受惠。在主要用户组中列出的调查人员将大约每天使用该工作站。该仪器将支持主要用户群体的研究领域包括细菌学、基因表达转录后控制、寄生虫学、分子/应用病毒学和药物发现。该工作站也将提供给学院和大学的其他研究小组使用。该工作站将由首席研究员监督和管理，作为微生物学大楼新多用户数据成像设施的核心。PI将监督操作和正确使用工作站的指导，监测使用情况，并与其他CSU研究人员制定未来的共享协议。将提供ImageQuant， Excel和其他适用软件的培训，以确保所有研究小组都能充分利用该设备。“台风三重奏”的收购不仅将提高用户组的整体生产力，而且还将允许开发新的实验方法，如果没有这种仪器，这些方法将是不可行的。购买台风三人成像仪将对科罗拉多州立大学微生物学、免疫学和病理学部门正在进行的广泛研究产生巨大影响。最重要的是，它将通过提高数据采集的速度和灵敏度，促进许多调查传染病的项目，包括结核病、麻风病、各种病毒（长曲棍球病毒、艾滋病病毒、登革热病毒、西尼罗河病毒）和朊病毒疾病。

DESCRIPTION (provided by applicant): This proposal requests funds to purchase a Typhoon Trio Imager Workstation. This imaging workstation allows for highly sensitive, quantitative data imaging analysis via six different scanning modes - four color fluorescence, phosphorimaging and chemiluminescence detection in a single high throughput system. No other comparable unit is currently available on the main campus of Colorado State University and the user group is currently relying on an outdated and overused Storm phosphorimager located 5 minutes away in the MRB building in a different college of the university. The unit will primarily serve the imaging needs of 65 faculty members of the Department of Microbiology, Immunology and Pathology which had extramural research funding in 2005 of over $38 million. Since the technologies supported by the Imaging Workstation have become commonplace in molecular biology, the unit will benefit the entire department. Investigators listed in the major user group will use the workstation on approximately a daily basis. The areas of research of the major user group that will be supported by the instrument include bacteriology, post-transcriptional control of gene expression, parasitology, molecular/applied virology and drug discovery. The workstation will also be made available to other research groups in the college and university. The workstation will be supervised and managed by the principal investigator as the centerpiece of a new multi-user data imaging facility in the Microbiology Building. The PI will oversee operation and instruction on the proper use of the workstation, monitoring usage, and developing future sharing agreements with other CSU researchers. Training in ImageQuant, Excel and other applicable software will be provided in order to ensure the device will be used to its full potential by all research groups. The acquisition of the Typhoon Trio will not only increase the overall productivity of the user group, but will also allow the development of novel experimental approaches that would not be feasible without such an instrument. Relevance The purchase of a Typhoon Trio Imager will have a huge impact on the wide range of ongoing research in the Microbiology, Immunology and Pathology Department at Colorado State University. Most significantly, it will facilitate many programs investigating infectious diseases including tuberculosis, leprosy, various viruses (LaCrosse, HIV, Dengue, West Nile Virus), and prion disease by increasing both the speed and sensitivity of data acquisition.