RNASE P

这个子项目是利用由NIH/NCRR资助的中心拨款提供的资源的许多研究子项目之一。子项目和调查员(PI)可能从另一个NIH来源获得了主要资金，因此可能会出现在其他CRISE条目中。列出的机构是针对中心的，而不一定是针对调查员的机构。酵母核RNase P负责前体tRNAs 5‘端的成熟。有几条证据使我们相信RNaseP有额外的底物，我们最近确定了几个候选者。目前正在努力确定已知核糖核酸酶P核心之外的相互作用伙伴。使用新的亲和标签，我们能够快速分离全酶，并有望找到在最初艰难的纯化过程中丢失的相互作用蛋白，这导致了RNaseP核心的鉴定。这些相互作用的伙伴将被调查他们在处理我们实验室最近确定的新的候选衬底中所扮演的角色。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The yeast nuclear RNase P is responsible for the maturation of the 5' end of precursor tRNAs. There are several lines of evidence that lead us to believe that RNase P has additional substrates and we have recently identified several candidiates. Work to attempt the identification of interacting partners outside the known core of RNase P is being carried out. Using novel affinity tags we are able to rapidly isolate the holoenzyme and expect to find interacting proteins that were lost during the initiual arduous purification that led to the identification of the RNase P core. These interacting partners will be investigated for their roles in the processing of novel substrate candidates recently identified in our lab.