STRUCTURAL STUDIES OF ROTAVIRUS POLYMERASE VP1

该子项目是利用NIH/NCRR资助的中心赠款提供的资源的许多研究子项目之一。子项目和研究者（PI）可能从另一个NIH来源获得主要资金，因此可以在其他CRISP条目中表示。所列机构为中心，不一定是研究者所在机构。引起儿童急性胃肠炎的轮状病毒作为模型系统和病原体已被广泛研究。病毒RNA的转录（从dsRNA合成（+）-链）和复制（从（+）-链ssRNA合成（-）-链）均由轮状病毒聚合酶VP 1进行。（-）-链RNA的体外复制需要核心晶格蛋白VP 2的存在。确定导致（-）-链病毒RNA合成的事件对于理解轮状病毒在宿主细胞中的一般复制，特别是基因组复制和衣壳组装的偶联至关重要。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Rotaviruses, which cause acute gastroenteritis in children, have been extensively studied both as model systems and as pathogens. The transcription ((+)-strand synthesis from dsRNA) and replication ((-)-strand synthesis from (+)-strand ssRNA) of viral RNAs are both carried out by rotavirus polymerase VP1. in vitro replication of (-)-strand RNA requires the presence of the core lattice protein VP2. Determining the events leading to the synthesis of the (-)-strand viral RNA is essential to understanding the replication of rotavirus in host cells in general, and the coupling of genome replication and capsid assembly in particular.