PREPARATION AND DISTRUBUTION OF ADULT STEM CELLS

描述（由申请人提供）：本提案的总体目标是建立一个成体干细胞（MSCs）的制备、质量测试和向多个研究人员分发的中心。这些细胞吸引了越来越多的关注，因为它们很容易从病人身上分离出来，在培养中扩增，并进行基因工程改造。它们可以分化成多种细胞系，最近报道在动物模型中对成骨不全、脊髓损伤、中风和心肌病等疾病有一定疗效。该中心将解决一个阻碍msc研究的关键问题；许多研究人员在获得细胞的标准化制备时遇到的困难。另外84名调查人员为目前的申请提供了支持信，这一事实反映了迫切需要一个在此提议的这种中心。这个名单基本上包括了这个快速发展领域的所有主要研究人员。具体目标是建立一个中心，该中心将：1)准备持续供应的人类间充质干细胞，这些干细胞经过彻底的质量测试，并根据要求分发给多个机构的其他研究人员进行细胞研究；2)准备类似的持续供应的大鼠间充质干细胞，分发给多个机构的研究人员；3)从其他机构研究者送来的人骨髓抽液中制备骨髓间充质干细胞，并将经质量检测的骨髓间充质干细胞返回给相同的研究者。此外，对其他机构研究人员制备的间充质干细胞进行质量检测；4)利用最近在细胞上发现的一系列表面表位的抗体，发展分离和表征人间充质干细胞的改进方法。

DESCRIPTION (provided by applicant): The overall aim of this proposal is to establish a Center for preparation, quality testing, and distribution to multiple investigators of the adult stem cells referred to as MSCs. The cells have attracted increasing attention because they are readily isolated from the patient, expanded in culture, and genetically engineered. They can differentiate into multiple cell lineages and have recently reported to have some efficacy in animal models for diseases such as osteogenesis imperfecta, spinal cord injury, stroke, and cardiomyopathies. The Center will address a critical problem that has delayed research with MSCs; the difficulties that many investigators have encountered in obtaining standardized preparations of the cells. The urgent need for a Center of the kind proposed here is reflected by the fact that 84 other investigators have provided Letters of Support for the present application. The list includes essentially all the leading investigators in this rapidly growing field. The Specific Aims are to establish a Center that will: 1) prepare a continuous supply of human MSCs that are thoroughly quality tested and distribute them on request to other investigators at multiple institutions for research on the cells; 2) prepare a similar continuous supply of rat MSCs for distribution to investigators at multiple institutions; 3) prepare MSCs from human bone marrow aspirates sent by investigators from other institutions and return the quality-tested MSCs to same investigators. Also, carry out quality testing of MSCs prepared by investigators at other institutions; and 4) develop improved methods of isolating and characterizing human MSCs using the antibodies to a series of surface epitopes that have recently been identified on the cells.