Core--Genomics

描述(申请人提供)：图拉氏方济氏菌是一种高度传染性的病原体，可通过气雾剂途径获得。因此，它已被归类为A类 由疾病预防控制中心选择代理。目前还没有获得许可的疫苗来预防这种病毒的感染。 有机体。基因组方法在很大程度上依赖于微阵列的开发和分析，将是解开关于有机体的毒力以及宿主在感染期间的反应的谜团的关键。该核心将侧重于支持PO1应用中的所有项目，目标如下：目标1是构建和利用F.tularensis Schu4寡核苷酸微阵列；以及，目标2是为项目项目提供DNA测序、寡核苷酸生产和其他核酸检测/分析功能(例如实时聚合酶链式反应和台风成像系统)的支持服务。在这一核心的支持下，各个项目将在弗朗西斯氏菌的发病机制和免疫领域带来新的发现。

DESCRIPTION (provided by applicant): Francisella tularensis is a highly infectious pathogen that can be acquired by the aerosol route. As such, it has been classified as a Category A select agent by the CDC. No licensed vaccine is available for protection against infection by this organism. Genomic approaches, relying heavily on microarray development and analysis, will be key to unlocking the mysteries concerning the virulence of the organism, as well as the response of the host, during infection. This Core will be focused on support for all of the Projects in this PO1 application with the following aims: Aim 1 is construction and utilization of a F. tularensis Schu4 oligonucleotide microarray; and, Aim 2 is support services to the program project for DNA sequencing, oligonucleotide production, and other nucleic acid detection/analytical functions (e.g., Real Time PCR and Typhoon Imaging system). With the support of this Core, the individual projects will lead to novel discoveries in the fields of Francisella pathogenesis and immunity.