Point Source Ozonation to Minimize Antibiotic Resistance

描述(由申请人提供)：在这个第二阶段的项目中，BlueInGreen，LLC将建造一个过饱和溶解臭氧(HYDOZTM)装置，部署在阿肯色州斯普林代尔的Springdale污水处理设施(SWWTF)的大型中试研究中。此外，BlueInGreen将与阿肯色大学合作，在实验室和中试研究中测量HYDOZ臭氧氧化去除废水中难降解微生物和稳定化学污染物的能力。第一阶段的结果表明，与传统的臭氧处理系统相比，HYDOZ臭氧处理在实验室规模上的效率和有效性都有所提高。第二阶段的研究将集中在使用标准的氯基消毒方法不能从废水中去除的稳定污染物和难降解微生物。我们将展示使用HYDOZ在集中式废水处理设施中处理废水在经济和技术上是一种可行的方法，可以防止药物残留物和抗药性微生物向环境释放。该项目直接实现了国家环境健康科学研究所的使命，即减少人类疾病和功能障碍对环境的负担，并将展示HYDOZ臭氧氧化处理废水的效果和效率。第二阶段的具体目标是：“目标1：比较HYDOZ与标准消毒/去污技术(BlueInGreen)的成本和效果。里程碑1：HYDOZ系统的资本成本和运行成本优于氯基消毒系统，根据标准出水水质参数，HYDOZ系统在废水处理方面优于氯基消毒系统。”目的：研究HYDOZ对废水中多种化学污染物(BlueInGreen)的破坏作用。里程碑2：HYDOZ臭氧氧化系统将使废水流出物中至少六种化学污染物的浓度降低至少90%。目标3：研究HYDOZ对废水中的质粒和难降解微生物的破坏(阿肯色大学)。里程碑3：HYDOZ臭氧氧化系统将使废水中的质粒和难降解微生物的浓度至少降低90%。 与公众健康相关：该项目的目标是使用过饱和溶解臭氧(HYDOZTM)系统从废水中去除稳定的化学/药物残留物和难降解的微生物。成功地减少这些污染物在水环境中的出现将减少对生态系统和人类的长期影响，并减少抗药性微生物的传播。该设备的广泛使用将大大有助于减少人类生态系统中的抗药性病原体，并有助于维持具有重要医学意义的抗生素的疗效。

DESCRIPTION (provided by applicant): In this Phase II project, BlueInGreen, LLC will build a hypersaturated dissolved ozone (HYDOZTM) unit to deploy in a large pilot-scale study at Springdale Wastewater Treatment Facility (SWWTF) in Springdale, Arkansas. Furthermore, BlueInGreen will collaborate with the University of Arkansas to measure the ability of HYDOZ ozonation to remove refractory microbes and stable chemical contaminants from wastewater effluent in both lab-scale and pilot- scale studies. Phase I results demonstrated the increased efficiency and effectiveness of HYDOZ ozone treatment at bench scale compared to conventional ozonation systems. Phase II research will focus on stable contaminants and refractory microbes that are not removed from wastewater using standard chlorine-based disinfection. We will show that the use of the HYDOZ to treat wastewater at centralized wastewater treatment facilities is a viable method economically and technologically to prevent the release of pharmaceutical residuals and resistant microbes to the environment. This project directly addresses the mission of the National Institute of Environmental Health Sciences to reduce the burden of human disease and dysfunction from the environment and will demonstrate both the efficacy and efficiency of HYDOZ ozonation of wastewater effluent. The specific Phase II objectives are: " Objective 1: Compare cost and efficacy of the HYDOZ to standard disinfection/decontamination technology (BlueInGreen). Milestone 1: The capitol and operating cost of HYDOZ systems is superior to chlorine-based disinfection systems, and based on standard effluent quality parameters, the HYDOZ systems are superior to chlorine-based disinfection systems for treatment of wastewater effluent. " Objective 2: Study HYDOZ destruction of a range of chemical contaminants in wastewater effluent (BlueInGreen). Milestone 2: The HYDOZ ozonation systems will cause a minimum 90% reduction in concentration of at least six chemical contaminants from wastewater effluent. " Objective 3: Study HYDOZ destruction of plasmids and refractory microbes in wastewater effluent (University of Arkansas). Milestone 3: The HYDOZ ozonation systems will cause a minimum 90% reduction in concentration of plasmids and refractory microbes from wastewater effluent. PUBLIC HEALTH RELEVANCE: The goal of this project is to use the hypersaturated dissolved ozone (HYDOZTM) system to remove stable chemical/pharmaceutical residuals and refractory microbes from wastewater. Successful reduction of the occurrence of these contaminants in the aqueous environment will reduce long-term impacts on ecosystems and humans, and reduce the spread of antibiotic resistant microbes. The widespread use of this device will significantly contribute to the reduction of antibiotic resistant pathogens in the human ecosystem and help maintain the efficacy of medically important antibiotics.