Translational Systems

翻译系统核心（核心C）将与项目负责人（哈蒙德、Insel和 Montminy）、核心A（Feramisco）和核心B（Miyanohara）。此共享资源旨在提供程序 科学家，在一个单一的设施，具体的技术，是劳动密集型，高度专业化，而不是一般 可用于单个实验室且昂贵。 该核心提供的特定翻译系统包括详细的心血管生理学研究， 开发心血管疾病模型和病毒载体基因转移方法。生理 评估将包括：1）经胸超声心动图，以评估心腔大小、壁厚，以及 2）评估心脏的收缩功能，包括测量心脏收缩末期功能; 压力-容积关系; 3）离体灌注心脏的研究，这将使更精细和具体的 在与复杂反射激活和肾上腺素能激活分离的受控环境中测量LV收缩力 与手术准备相关的激活; 4）遥测以评估动态血压和ECG 啮齿动物 除了为所有计划科学家提供这些研究外，翻译系统核心还将提供一个 高成本效益的手段进行这样的研究在一个集中和设备齐全的位置，规避 需要各参与实验室的重复努力。通过巩固生理模型 开发和评估以及病毒基因转移，节省的成本将是相当可观的。

The Translational Systems Core (Core C) will interface with the Project Leaders (Hammond, Insel, and Montminy), Core A (Feramisco) and Core B (Miyanohara). This shared resource is designed to provide Program scientists, in a single facility, specific techniques that are labor intensive, highly specialized, not generally available to individual laboratories and expensive. The specific translational systems provided by this Core include detailed cardiovascular physiological studies, the development of cardiovascular disease models, and virus vector gene transfer methods. Physiological assessment will include: 1) Transthoracic echocardiography to evaluate cardiac chamber size, wall thickness, and function in vivo; 2) Assessment of contractile function of the heart, including measurement of the end-systolic pressure-volume relationship; 3) The study of isolated perfused hearts, which will allow more refined and specific measurements of LV contractility in a controlled setting isolated from complex reflex activation and adrenergic activation associated with surgical preparations; 4) Telemetry to assess blood pressure and ECG in ambulatory rodents. In addition to providing these studies for all Program scientists, the Translational Systems Core will provide a highly cost efficient means to conduct such studies in a centralized and well-equipped location, circumventing the need for redundant efforts of individual participating laboratories. By consolidating physiological model development and assessment and viral gene transfer, the cost savings will be considerable.