Sympathochromaffin cell culture

核心F将为研究者提供交感嗜铬细胞培养和相关的培养细胞系统， 这个计划的项目。核心F将常规制备原代培养物，包括 来自牛肾上腺髓质的交感嗜铬细胞、上级颈神经节细胞和相关细胞。 将培养神经内分泌细胞系，包括大鼠肾上腺髓质PC 12细胞和小鼠 垂体AtT-20细胞和实验目的所需的其他相关细胞。这个核心会准备好 维护和培养细胞，供项目负责人监督实验。制备的细胞的均匀性 该计划项目的相同程序将允许整合科学成果， 多个效应器的作用，经历胞吐作用的血压调节。核心F还将 提供用影响血压调节剂的胞吐作用的试剂处理的细胞。细胞将经历 用尼古丁、毛喉素、catestatin、NPY、蛋白酶抑制剂和相关试剂治疗， 影响血压的分泌性神经效应物的功能。细胞也将经历均匀的 用具有与人高血压相关的变异SNP序列的所选基因转染，基于 分子遗传学数据细胞培养核心将促进 项目和本项目的核心。有明确的分泌细胞制备物， 跨几个项目将提高我们的能力，合作，整合结果的凝聚力的假设， 结论，并共同出版。这些都是评价多种免疫调节因子协同调节的重要因素。 参与血压调节的交感神经效应分子。

Core F will provide sympathochromaffin cell cultures and related culture cell systems for investigators of projects of this program. Core F will prepare, on a routine basis, primary cultures that include sympathochromaffin cells from bovine adrenal medulla, superior cervical ganglion cells, and related cells. Neuroendocrine cell lines will be cultured and will consist of rat adrenal medullary PC12cells, and mouse pituitary AtT-20 cells, and other related cells required for experimental goals. This core will prepare, maintain, and grow cells for experiments supervised by project leaders. The uniformity of cells prepared with identical procedures for projects of this program will allow integration of scientific results with respect to the roles of multiple effectors that undergo exocytosis for the regulation of blood pressure. Core F will also provide cells treated with reagents that influence exocytosis of blood pressure regulators. Cells will undergo treatments with nicotine, forskolin, catestatin, NPY, protease inhibitors, and related reagents that modify functions of secreted neuroeffectors that influence blood pressure. Cells will also undergo uniform transfections with selected genes that possess variant SNP sequences related to human hypertension, based on molecular genetic data from the program. The cell culture core will promote synergistic interactions among projects and cores of this program project. Having well-defined secreting cell preparations that are uniform across several projects will enhance our ability to collaborate, integrate results in cohesive hypotheses and conclusions, and co-publish. These are important factors for evaluating the co-regulation of multiple sympathoeffector molecules that participate in blood pressure regulation.