CORE--Crystallographic data collection at the Advanced Photon Source

该核心项目的目的是在阿贡国家实验室先进光子源的工业大分子晶体学协会协作访问小组（IMCA-CAT）的设施中有效地获取和处理衍射数据。晶体学数据收集不应该成为解决真核蛋白中多个剪接位点结构问题的瓶颈。只要(a)收集日常数据的程序变得有效率和简单明了，数据收集就不会成为瓶颈；(b)选择多波长数据收集的峰值和拐点能量的程序是稳定的；(c)可获得非标准但有用的多波长或优化异常能量；(d)处理衍射数据的程序易于使用，能够跟上数据采集速率。在IMCA-CAT设施中，这些任务大部分已经完成了当前一轮结构基因组学项目中使用的吞吐量水平。随着不久的将来需求的增加，我们正在采取行动，从四个方面改善设施，以满足这一需求。具体来说，我们打算在IMCA-CAT的插入装置光束线上安装一个样品装载机器人系统，17-ID，并对该光束线上的单色仪、可视化光学系统和准直组件进行改进。在IMCA-CAT的弯曲磁体波束线17-BM上，我们计划安装一个准直镜，它应该能提供两倍以上的信噪比改善。随着多重剪接项目的进行，我们将为有效的数据采集和处理提供场地，并不断改进设施。多重剪接项目和IMCA-CAT的主要医药用户群体将从彼此的进步中受益。

The aim of this core project is to acquire and process diffraction data effectively and efficiently at the facilities of the Industrial Macromolecular Crystallography Association Collaborative Access Team (IMCA-CAT) at the Advanced Photon Source, Argonne National Laboratry. Crystallographic data collection should not be a bottleneck in the process of solving structures and identifying the structural implications of multiple splicing sites in eukaryotic proteins. Data collection will not become a bottleneck as long as (a) procedures for collecting routine data are rendered efficient and fool-proof; (b) procedures for choosing peak and inflection-point energies for multiwavelength data collection are stable; (c) access to non-standard but useful multiwavelength or optimized-anomalous energies is available; and (d) procedures for processing the diffraction data are easy to use and can keep up with the data acquisition rate. At the IMCA-CAT facilities, these tasks are for the most part already accomplished for the level of throughput used in the current round of structural genomics projects. As the demand increases in the near future, we are moving to improve the facilities in all four ways in order to address that demand. Specifically, we intend to install a sample-loading robotics system on IMCA-CAT's insertion-device beamline, 17-ID, and make improvements in the monochromator, the visualization optics, and the collimation assembly on that beamline. On IMCA-CAT's bending-magnet beamline, 17-BM, we plan the installation of a collimating mirror that should provide a better than twofold improvement in signal-to-noise ratios. As the multiple-splicing project proceeds,we will provide a site for effective data acquisition and processing, and make steady improvements in the facilities. The multiple-splicing project and IMCA-CAT's primary constituency of pharmaceutical users will benefit from one another's advancements.