Xenogen Rodent Imaging System

描述(由申请人提供)：本申请的目标是购买Xenogen IVIS光谱成像系统，以放置在加州大学伯克利分校的实验动物设施中。非侵入性成像技术的最新进展，如体内生物发光和荧光的高灵敏度检测，为癌症和代谢研究创造了新的机会，并有望在活体啮齿动物模型系统的背景下极大地提高我们对复杂细胞和分子相互作用的理解。Xenogen IVIS光谱成像系统可以对麻醉小鼠或大鼠发出的生物发光或荧光信号进行高分辨率、实时、三维图像采集。因此，该系统具有极强的通用性，可用于跟踪荧光/生物发光病原体和癌细胞的生长和扩散，并允许在体内实时检测基因活性、营养吸收和其他代谢过程。从单个动物顺序收集时间点数据的能力减少了所需的动物数量，并提供了关于基因表达和/或器官/肿瘤变化的实时更好控制的数据，以响应实验刺激。伯克利分校目前还没有这种类型的仪器。该仪器将(最初)服务于四个系中具有高度活跃的生物医学相关研究项目的八名研究人员的实验室，从事与癌症、免疫学、脂肪生成、能量平衡、脂肪代谢和环境介导性疾病的遗传效应相关的各种项目。这八名研究人员将使用生物成像仪：通过靶向癌细胞表面上调的碳水化合物对小鼠肿瘤进行成像；研究自然杀伤细胞和T细胞在对抗病毒和监测新生肿瘤中的功能；推进用于治疗神经退行性疾病的基因传递和干细胞疗法的分子工程；量化肥胖和瘦小RAG-1免疫损害小鼠的前列腺癌和乳腺癌细胞系的生长和转移；评估脂肪酸或胆汁酸调节的转录因子，如LXR和PPAR家族成员的激活模式和持续时间；监测基因表达，以评估体内的启动子反应元件；监测对金属暴露(铜、锌和铁)的反应；监测体内感染的进展；评估与维甲酸状态改变相关的基因表达变化。这一仪器的提供无疑会刺激校园和该地区的其他人使用它。

DESCRIPTION (provided by applicant): The goal of this application is to enable the purchase of a Xenogen IVIS Spectrum Imaging System for placement in the laboratory animal facility of the UC-Berkeley campus. Recent advances in non-invasive imaging technologies, such as high-sensitivity detection of in vivo bioluminescence and fluorescence, have created novel opportunities for cancer and metabolic research and promise to greatly enhance our understanding of complex cellular and molecular interactions in the context of living rodent model systems. The Xenogen IVIS Spectrum Imaging System allows for high-resolution, real time, three- dimensional image acquisition of bioluminescent or fluorescent signals emanating from anesthetized mice or rats. Thus, the system is extremely versatile and can be used to track growth and spread of fluorescent/bioluminescent pathogens and cancer cells, and allows for real-time in vivo detection of gene activity, nutrient uptake, and other metabolic processes. The ability to sequentially gather time-point data from a single animal decreases the number of animals needed, and provides better-controlled data with respect to gene expression and/or organ/tumor changes in real time in response to experimental stimuli. No instrument of this type exists currently on the Berkeley campus. This instrument would serve (initially) the labs of eight investigators with highly active biomedically relevant research programs in four departments, working on a variety of projects related to cancer, immunology, adipogenesis, energy balance, lipid metabolism, and genetic effects on environmentally mediated diseases. The eight investigators would use the bioimager to: image tumors in murine models by targeting carbohydrates up regulated on the surfaces of cancer cells; study functions of natural killer cells and T cells in fighting viruses and in surveillance of nascent tumors; advance molecular engineering of gene delivery and stem cell therapies for the treatment of neurodegenerative diseases; quantify growth and metastasis of prostate and breast cancer cell lines in obese and lean Rag-1 immuno-compromized mice; assess activation patterns and duration of fatty acid or bile acid regulated transcription factors, such as LXR and PPAR family members; monitor expression of genes to evaluate promoter response elements in vivo; monitor responses to metal exposure (copper, zinc and iron); monitor progress of infections in vivo; and assess gene expression changes related to alterations in retinoid status. Availability of this instrument would undoubtedly stimulate its use by others on campus and in the area.