Functional Mapping of Ethanol Avoidance in Mouse Pain

当比较两个品系的小鼠时，观察到相反的乙醇自我给药行为：C57 BL/6 J x NZB/B1 NJ F1杂种（B6 xNZB）在经历高浓度乙醇后避免乙醇（避免者）。 anol和C57 BL/6 J x FVB/NJ F1杂种（BGxFVB）则不存在（revelers）。这个最重要的假设是 我们的建议是，这些杂交种对厌恶的敏感性不同，但不是奖励或抗焦虑的， 乙醇和FosB和AFosB在特定细胞类型和大脑区域的差异水平反映了经验 自愿乙醇自我管理的依赖性差异。对厌恶、奖励和 乙醇的抗焦虑特性将通过采用以下试验来确定：条件味觉保存， 条件位置偏爱、改良两瓶选择和高架十字迷宫。去定义神经- 通过酒精回避和狂欢参与的回路，FosB和AFosB免疫反应性将在 乙醇经历了混合物。FosB和AFosB表达神经元的神经细胞类型也将被确定。 地雷。FosB/AFosB阳性细胞核的应变、剂量和数量（每个脑区域和细胞类型）的影响将 被确定。这些实验将有助于了解酒精的使用和滥用。

Opposing ethanol self-administration behaviors are observed when comparing two strains of mice: C57BL/6J x NZB/B1NJ F1 hybrids (B6xNZB) avoid ethanol (avoiders) after experience with high concentrations of eth- anol and C57BL/6J x FVB/NJ F1 hybrids (BGxFVB) do not (revelers). The overarching hypothesis of this proposal is that these hybrids differ in sensitivity to the aversive, but not rewarding or anxiolytic, properties of ethanol and differential levels of FosB and AFosB in specific cell types and brain regions reflects experience dependent differences in voluntary ethanol self-administration. Hybrid sensitivity to aversive, rewarding, and anxiolytic properties of ethanol will be determined by employing the following tests; conditioned taste aver- sion, conditioned place preference, modified two bottle choice, and elevated plus maze. To define neuro- circuits engaged by ethanol avoidance and revelry, FosB and AFosB immunoreactivity will be measured in ethanol experienced hybrids. Neuronal cell types of FosB and AFosB expressing neurons will also be deter- mined. Effects of strain, dose, and quantity of FosB/AFosB positive nuclei (per brain region and cell type) will be determined. These experiments will contribute to the understanding of alcohol use and abuse.