Proteomics

在接下来的5年里，我们将进行一系列先进的数据采集， 为体内信号调节NR通路的“系统生物学”提供蛋白质组图谱。如此高的吞吐量 数据采集项目本身不可能作为R01机制，但积累的 数据将极大地刺激整个核受体科学领域的假设驱动研究。我们将 继续分离和鉴定小鼠组织中的辅调节因子（CoR）复合物，改进实验方案 用于从小鼠肝脏、脂肪细胞、子宫和脑组织中提取蛋白质， 分析，并从小鼠组织中分离和鉴定辅调节蛋白复合物用于质量分析 规格我们将对辅调节因子复合物的翻译后修饰（PTM）进行分析， 还将描绘生理信号传导后NR和CoR的协调PTM。

In the next 5 years of the NURSA project, we will carry out a series of advanced data acquisitions that will provide a proteomic atlas for the "systems biology" of signal-regulated NR pathways in vivo. Such highthroughput data acquisition projects are not themselves possible as R01 mechanisms, yet the accumulated data will greatly stimulate hypothesis-driven research in the entire field of nuclear receptor science. We will pursue the isolation and identification of coregulator (CoR) complexes in mouse tissues, improving protocols For generating protein extracts from mouse tissues of liver, adipocytes, uterus and brain for proteomic analyses, and isolating and identify coregulator protein complexes from mouse tissues for analysis by mass spec. We will carry out profiling of post-translational modifications (PTM) of coregulator complexes and we will also profile coordinated PTMs of NR and CoRs following physiologic signaling.