Microbicide Perfomance Analysis: Distribution, Drug Delivery and Pharmacodynamics

本项目是研究联盟项目“长效可接受的杀菌剂： 新的传递，活性和药效学。它将评估新的凝胶和环材料的传递 杀微生物剂对阴道腔和上皮的影响。新的体外和体内分析，以及数学 将开发凝胶分布、药物输送和艾滋病毒中和的模型。与当前一起 技术在我们实验室中运行，这套多样化的性能分析分析将提供 对研究联盟的项目1和3的反馈。体外分析和数学模型将 抗微生物活性成分的凝胶、环性质和效力与精液产生的程度有关 HIV在迁移到上皮细胞并启动传播过程之前被中和。当前版本和新版本 活体成像技术将绘制出人体阴道上皮被杀微生物剂凝胶的涂层。这 信息将帮助项目1开发新的凝胶和环，这些凝胶和环具有优化的阴道性能 分销和药物输送。与项目3的相互作用将把凝胶和环的生物物理性质联系起来 以衡量妇女对她们的感知和接受程度。项目1、2和3将共同创造新的 具有共同优化的性能以提供良好的抗艾滋病毒生物活性的杀微生物剂凝胶和环 传播和女性良好的行为可接受性。

This project is part (Project 2) of the research consortium project "Long Acting Acceptable Microbicides: Novel Delivery, Activity and Pharmacodynamics." It will evaluate new gel and ring materials for delivery of microbicides to the vaginal lumen and epithelium. New in vitro and in vivo assays, and mathematical models, of gel distribution, drug delivery and HIV neutralization will be developed. Together with current techniques operational in our laboratory, this diversified set of performance analysis assays will provide feedback to Projects 1 and 3 of the research consortium. The in vitro assays and mathematical models will relate gel and ring properties and potency of active microbicidal ingredients to the extent that semen borne HIV is neutralized before it migrates into epithelium and initiates the transmission process. Current and new in vivo imaging techniques will map the coating of human vaginal epithelium by microbicide gels. This information will help enable Project 1 develop new gels and rings with properties optimized for good vaginal distribution and drug delivery. Interactions with Project 3 will link biophysical properties of the gels and rings to measures of their perception and acceptability by women. Together, Projects 1, 2 and 3 will create new microbicide gels and rings with properties co-optimized to provide good biological activity against HIV transmission and good behavioral acceptability by women.