Flow Cytometry

流式细胞术核心的总体目标是有效地为CFAR成员提供 全面的流式细胞仪服务，包括设备、专业知识和培训，费用合理。 流式细胞术是一种独特而强大的技术，可以确定细胞的表型和功能 还允许通过细胞分选来分离定义的细胞群体。近几年来流量的使用 细胞分析在HIV研究中已变得更加突出，因为它是解剖 艾滋病毒与免疫系统细胞之间的相互作用已经发展起来。恒虚警流式细胞术 CORE于1990年开始运作，并在#年继续为艾滋病研究人员提供流式细胞仪服务 UAB社区。在上一次预算期间，82名个人研究人员使用了流式细胞仪核心， 包括28名艾滋病相关项目的CFAR成员。地芯中的仪器最近也 已经升级，现在使调查人员能够使用三束激光分析六种不同的参数 可能的发射波长为360 nm、407 nm、488 nm、528 nm、568 nm或633 nm。此外，CFAR流程 细胞仪核心现在还可以使用FACScan仪器进行五参数、三色分析。 流式细胞仪核心的具体目标是： 1.服务。为艾滋病中心成员提供细胞分选和流式细胞仪分析服务， 它们能够处理潜在的传染性物质。 2.教育和培训。为爱滋病中心会员提供有关 流式细胞术在艾滋病研究中的应用。 3.发展。流式细胞仪核心的一个主要目标是通过确保艾滋病 中心成员可以利用当前的技术进步并使用最先进的技术 在他们的研究活动中使用技术。 总体而言，CFAR流式细胞术核心为艾滋病提供关键资源、专业知识和支持 UAB的研究人员，并作为许多科学互动的协调中心。

The overall objective of the Flow Cytometry Core is to efficiently provide CFAR members with comprehensive flow cytometry services, including equipment, expertise and training at a reasonable cost. Flow cytometry is a unique and powerful technology for determining the phenotype and function of cells and also allows defined populations of cells to be isolated by cell sorting. In recent years the use of flow cytometric analysis in HIV research has become even more prominent as new approaches for dissecting the interactions between HIV and cells of the immune system have been developed. The CFAR Flow Cytometry Core began operation in 1990 and continues to provide flow cytometry services to the AIDS researchers in the UAB community. During the last budget period 82 individual investigators used the flow cytometry core, including 28 CFAR members for AIDS-related projects. The instrumentation in the core has also recently been upgraded and now enables investigators to analyze six different parameters using three laser beams with possible emissions at 360 nm, 407 nm, 488nm, 528 nm, 568 nm, or 633nm. In addition the CFAR Flow Cytometry Core now also has available a FACScan instrument for five parameter, three color analysis. The specific aims of the flow cytometry core are: 1. Service. To provide AIDS Center members with cell sorting and analytical flow cytometry services, which are capable of handling potentially infectious materials. 2. Education and Training. To provide AIDS Center members with up to date information regarding the use of flow cytometry in AIDS research. 3. Development. A major goal of the flow cytometry core is to address users' needs by ensuring that AIDS Center members can take advantage of current technological advances and employ state of the art techniques in their research activities. Overall the CFAR Flow Cytometry Core provides key resources, expertise and support to AIDS investigators at UAB and acts as a focal point for numerous scientific interactions.