Virtual microscopy of zebrafish as a community resource

描述（由申请人提供）：NIH赞助的斑马鱼和水生模型工作组建议创建基于网络的解剖学，组织学和病理学地图集作为社区资源。主要研究人员建议为斑马鱼的基于网络的虚拟/显微镜和3D生成和复制参考载玻片。该地图集将通过俄勒冈州大学的斑马鱼信息网（ZFIN）（http：//zfin.org/ZFIN/）提供。在虚拟显微镜中，任何具有Web浏览器和快速互联网连接的计算机都可以用作显微镜，使用远程服务器上的注释虚拟载玻片文件。虚拟幻灯片是GB大小的高放大率图像块或条带的压缩合成物;根据用户选择的视野和放大率将图像包发送给远程用户。在3D解剖图谱中，可以查看和旋转选定的解剖结构。图谱将包括正常和一些异常的胚胎、幼虫、青少年和成年人。斑马鱼的实验特征使其成为功能基因组学的首要模型。高通量突变体、反义敲除（“morphants”）、荧光转基因和化学筛选已经产生了数千种潜在的人类疾病模型，这些模型在生长和发育的各个阶段影响各种生物学功能。一个基于网络的，全面的正常斑马鱼参考图谱将大大促进这项工作。对这类地图集进行远程注释和获取这类地图集将有助于地图集作为一种资源的发展，也有助于单独和合作调查。具体目标1是生成一个注册的系列，染色石蜡和塑料切片的正常斑马鱼胚胎，幼虫，青少年和成年人。具体目标2是扫描这些切片以创建虚拟载玻片。将生成说明性的注释字段对。具体目标3是在斑马鱼胚胎，幼虫，青少年和成年人的3D重建数字轮廓组织学可识别的结构。由此产生的基础设施将与其他模型系统的基础设施相辅相成、相互适应并相互协调。该地图集将支持多个学科的斑马鱼社区，并解决几乎所有NIH研究所的任务。

DESCRIPTION (provided by applicant): NIH-sponsored zebrafish and Aquatic Models working groups have recommended the creation of web based anatomy, histology and pathology atlases as a community resource. The principal investigators propose to generate and digitize reference glass slides for web-based virtua/microscopy and 3D at as of the zebrafish. This atlas will be available through the Zebrafish Information Network (ZFIN) of the University of Oregon (http://zfin.org/ZFIN/). In virtual microscopy, any computer with a web browser and fast internet connection can function as a microscope, using annotated virtual slide files on a remote server. Virtual slides are GB size, compressed composites of high magnification image tiles or strips; image packets are sent to the remote user according to user-chosen field-of-view and magnification. In the 3D anatomy atlas, selected anatomical structures can be viewed and rotated. The atlases will include normal, and some abnormal, embryos, larvae, juveniles and adults. The experimental features of the zebrafish have made it a premier model for functional genomics. High-throughput mutant, antisense knockdowns ("morphants"), fluorescent transgenic, and chemical screens have already yielded thousands of potential models of human disease affecting a variety of biological functions at a variety of stages of growth and development. A web-based, comprehensive reference atlas of normal zebrafish would greatly facilitate this work. Remote annotation of and access to such atlases will facilitate the growth of the atlas as a resource, as well as individual and collaborative investigations. Specific Aim 1 is to generate a registry of serial, stained paraffin and plastic sections of normal zebrafish embryos, larvae, juveniles, and adults. Specific aim 2 is to scan these sections to create virtual slides. Illustrative pairs of annotated fields will be generated. Specific Aim 3 is to digitally outline histologically identifiable structures in zebrafish embryos, larvae, juveniles, and adults for 3D reconstructions. The resultant infrastructure will be complementary to, adaptable to, and coordinated with those of other model systems. The atlas will support the zebrafish community across multiple disciplines, and address the missions of nearly all Institutes of the NIH.