Groundwork for a Synchrotron MicroCT Imaging Resource for Biology (SMIRB)

摘要 我们要求改建和装修补充资金，使我们能够完成关键的主要 我们的项目的目标，为生物学同步微CT资源的基础。启用的工作 将扩大对这种新技术在各种模型中的潜在影响的理解 系统和实验问题。具体来说，这笔资金将允许我们搬进一个新的房间 最近由宾夕法尼亚州立大学医学院授予，这将使我们能够适应合作 人员计算空间，以及两台microCT机。新的“生产”microCT 成像系统将创造一种方法，继续扫描试点样本的范围合作者， 我们继续对第一个系统进行不间断的“开发”工作，以增加视场， 吞吐量和图像质量。目前迫切需要补充资金是由于 最近收到一个新的12毫米视场系统，大大增加了样品的类型， 可以使用我们的新技术进行成像-去年的系统进步使之成为可能 补充.简而言之，3D图像的泛细胞、全体积性质将允许前所未有的 对所有生物体的生命和组织结构多样性进行表征的范围和深度。的 补充将大大提高我们的能力，通过创造新的空间生物学的新领域， 用于其它成像模式如免疫组织化学的显微解剖和解剖锚， 免疫荧光和MRI，以及转录组学，蛋白质组学，代谢组学和其他 分子组学数据。

Abstract We request Alternation and Renovation supplementary funds to allow us to accomplish critical primary goals of our project, Groundwork for a Synchrotron MicroCT Resource for Biology. The enabled work will broaden understanding of the potential impact of this new technology across a variety of model systems and experimental questions. Specifically, the funds would allow us to move into a new room recently awarded by Penn State College of Medicine that will allow us to accommodate collaborative computational space for personnel, as well as two microCT machines. The new “production” microCT imaging system will create a way to continue scanning pilot samples for a range of collaborators while we continue uninterrupted “developmental” work on the first system to increase field-of-view, throughput, and image quality. The urgency for supplementary funds at this time is caused by the recent receipt of a new 12 mm field-of-view system that greatly increases the types of samples that can be imaged using our new technology – a system advance made possible by last year’s supplement. In short, the pan-cellular, full volume nature of the 3D images will allow unprecedented scope and depth of characterization of the diversity of life and tissue structure across all organisms. The supplement will greatly increase our ability to advance the new field of spatial biology by creating new microanatomic and anatomic anchors for other imaging modes such as immunohistochemistry, immunofluorescence, and MRI, and for transcriptomics, proteomics, metabolomics and other molecular “-omics” data.