CORE-- Imaging and Cytometry Facilities Core

描述（由申请人提供）：成像和流式细胞术中心（ICFC）的使命是支持EHS中心成员建立的、同行评审的、资助的研究项目，通过向个体研究者提供显微镜和流式细胞术方面的当代技术、仪器和服务，费用很少或没有。ICFC通过提供必要的仪器，培训和专业知识来提高中心成员的研究生产力，以成功地将显微镜，流式细胞仪和成像技术应用到他们的个人和合作研究计划中。在上一个供资期间，30名调查员利用国际儿童基金会的服务和设施发表了83份手稿，并获得了29笔赠款（新的和续发的），另有3笔有待利用国际儿童基金会使用的数据。获得这些技术对于从事环境、细胞和分子毒理学研究，特别是信号转导研究至关重要。ICFC在以下领域促进了多学科合作研究： 外源性物质对多种细胞/器官系统的影响、重金属对细胞功能和信号传导的影响、外源性物质代谢酶基因表达的调节、响应于毒性损伤的DNA修复、细胞信号传导途径的调节、细胞对氧化应激的反应以及与哮喘易感性相关的T细胞表型。ICFC将吸引新用户和新中心成员。自上次竞争性续延以来，有16个新用户（均为中心成员）使用了国际鱼类养殖中心的服务/设施。ICFC通过提供几乎不需要调查员投入初始时间或金钱的服务，促进了对调查员个人和合作研究方案采用新方法。ICFC提供个人研究者无法获得的技术和仪器，并努力引入新技术以满足中心研究者的需求。先进的设备由专家核心人员维护和操作，他们为使用该设施的EHS中心成员提供服务，指导和培训。研究人员可以接受光，共聚焦和荧光显微镜，免疫细胞化学，流式细胞术和细胞分选，以及彗星试验的实践培训。 ICFC还维护用于样品分析的设备和仪器（例如，光度计、荧光计、光度计、Agilent Bioanalyzer）和数据采集（例如，凝胶记录系统、胶片显影剂、扫描激光密度计）。ICFC的目标是在一个功能多用户环境中获取和维护这些工具和服务，而单个调查人员通常无法获得这些工具和服务。

Description (provided by applicant): The mission of the Imaging and Cytometry Facility Core (ICFC) is to support established, peer-reviewed, funded research programs of EHS Center members by providing access to contemporary technology, instrumentation, and services in microscopy and flow cytometry at little or no cost to individual investigators. The ICFC enhances the research productivity of Center members by providing the instrumentation, training, and expertise necessary to successfully adopt microscopic, flow cytometric, and imaging techniques into both their individual and collaborative research programs. During the past funding period, the 30 investigators utilizing ICFC services and facilities have published 83 manuscripts and received funding for 29 grants (new and renewal), with another 3 pending, utilizing data generated through ICFC use. Access to these technologies is essential for pursuing studies in environmental, cellular, and molecular toxicology, especially those in signal transduction. The ICFC has fostered multi-disciplinary collaborative research in the areas of xenobiotic effects on a variety of cell/organ systems, the effects of heavy metals on cell function and signalling, the regulation of xenobiotic metabolizing enzyme gene expression, DNA repair in response to toxic insult, regulation of cellular signalling pathways, cellular responses to oxidative stress, and T-cell phenotype association with asthma susceptibility. The ICFC are to attract new users and new Center members. Since the last competitive renewal, 16 new users, all Center members, have utilized the ICFC services/facilities. The ICFC facilitates the introduction of new approaches to investigators' individual and collaborative research programs through the provision of services requiring little initial input of time or money on the part of the investigator. The ICFC provides access to technologies and instrumentation that would otherwise be unavailable to individual investigators, and strives to introduce new technologies in response to the Center investigator needs. Advanced equipment is maintained and operated by expert core personnel, who provide service, guidance, and training to EHS Center members using the facility. Investigators can receive hands-on training in light, confocal, and fluorescent microscopy, immunocytochemistry, flow cytometry and cell sorting, and the comet assay. The ICFC also maintains equipment and instrumentation for sample analysis (e.g., spectrophotometers, fluorometer, luminometer, Agilent Bioanalyzer) and data acquisition (e.g., gel documentation system, film developer, scanning laser densitometer). The goal of the ICFC is to acquire and maintain these instruments and services in a functional multi-user environment normally unavailable to individual investigators.