Scattering Core

12)小角度散射核（Trewhella。主任） 概述 我们的小角散射核心提供最先进的仪器和专业知识， 获取和解释蛋白质、蛋白质复合物和 溶液中的组件，以及用于蛋白质生产和生物化学的必要相关设施 和生物物理表征。我们拥有X射线溶液散射的“内部”能力（在 犹他州和悉尼大学），我们将使用OPAL的中子散射设施 澳大利亚核科学与技术组织（ANSTO）的研究反应堆。 每一个蛋白质或蛋白质组装体的研究将需要广泛的表征首先与X射线 分散以确定系统满足溶液分散的严格要求： 单分散相同的颗粒和接近稀溶液条件或 在一定浓度范围内采集数据并外推至无限稀释条件。这项工作 将主要在犹他州大学进行，以促进与 艾滋病毒蛋白生物化学专业知识。一旦这些条件建立起来，我们就知道了 我们可以从X射线实验、中子衬度变化实验中对配合物进行靶向 可以使用悉尼大学的可用资源来计划和实施。

12) Small Angle Scattering Core (Trewhella. Director) Overview Our Small Angle Scattering Core provides state of the art instrumentation and expertise for the acquisition and interpretation of X-ray and neutron scattering data from proteins, protein complexes and assemblies in solution, as well as necessary associated facilities for protein production and biochemical and biophysical characterization. We have "in house" capabilities for X-ray solution scattering (at the University of Utah and University of Sydney), and we will use neutron scattering facilities at the OPAL Research Reactor at Australian Nuclear Science and Technology Organization (ANSTO). Each protein or protein assembly to be studied will require extensive characterization first with X-ray scattering to establish that the systems meet the stringent requirements for solution scattering: monodisperse identical particles and the ability to either approximate dilute solution conditions or to acquire data over a range of concentrations and extrapolate to the infinite dilution condition. This work will primarily be performed at the University of Utah to facilitate the close interactions required with the HIV protein biochemistry expertise. Once these conditions are established and we have learned all that we can from the X-ray experiments, neutron contrast variation experiments on the complexes targeted for study can be planned and implemented using the resources available at the University of Sydney.