SMALL ANGLE SCATTERING STUDIES OF CALCIUM BINDING PROTEINS & THEIR COMPLEXES

钙结合蛋白的小角散射研究

• 批准号: 6658761
• 负责人: JILL TREWHELLA
• 金额: $ 14.32万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-03-01 至 2003-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6658761
• 关键词: bioimaging /biomedical imaging; biomaterials; biomedical resource; minerals; proteins; radiography; structural biology

We propose to extend small-angle scattering studies of the interactions between the Ca2+ binding proteins calmodulin and troponin C and their regulatory targets. The systems we now wish to focus on have limited solubility resulting in the need for the most intense X-ray source available. A critical question to be answered is how well the previously used short peptide fragments have modeled interactions with intact target enzymes. The result will be a better understanding of the molecular basis for the Ca2+-dependent regulation in these important biochemical systems.

我们建议扩展小角散射研究的 钙调蛋白和肌钙蛋白之间的相互作用 C及其调控目标。 我们现在要关注的系统 具有有限的溶解度，导致需要最强烈的 X射线源可用。 一个需要回答的关键问题是， 先前使用的短肽片段已经模拟了 与完整的靶酶相互作用。 结果会更好 了解钙离子依赖性调节的分子基础 在这些重要的生化系统中。