CORE E

E.晶体筛选和晶体结构 主任：约翰·列侬叶博士 来自细菌中产生的蛋白质生产核心的蛋白质用于晶体结构分析， 被Se-Met标记以直接实现定相。对于那些在其他宿主中产生或最初由 合作者，其他的方法来定相，如通过多种同晶替代方法， 将在下面描述。主要目标是（1）开发结晶和添加剂筛 专门为蛋白质复合物配制，以优化获得衍射晶体的可能性，以及（2） 为了使用高通量结晶方法解析HIV辅助/调节蛋白的结构， 数据收集和结构确定。最终目标是解决艾滋病毒的结构 与细胞蛋白复合的辅助/调节蛋白，并通过使用合理的 方法，如本提案所述。

E. Crystal Screening and Crystal Structure Director: Joanne I. Yeh, PhD Proteins from the Protein Production Core produced in bacteria for crystal structure analysis will routinely be Se-Met labeled to directly enable phasing. For those produced in other hosts or initially provided by collaborators, additional approaches to phasing, such as via multiple isomorphous replacement methods as described below, will be used. The major objectives are (1) to develop crystallization and additive screens formulated specifically for protein complexes to optimize the probability of obtaining diffracting crystals and (2) to solve structures of HIV accessory/regulatory proteins using high-throughput approaches for crystallization, data collection, and structure determination. The ultimate goal is to solve the structures of HIV accessory/regulatory proteins in complexes with cellular proteins and to accomplish this by using rational approaches, as described throughout this proposal.