GENOMICS SHARED RESOURCE

AECC DMA测序共享资源成立于1992年，此后有了长足的发展 然后，特别是在上一个资助期，以满足AECC成员不断发展的科学需求 和程序。这个机构的新名称，基因组共享资源，反映了这种服务的扩展 和技术。该设施现在除了提供DMA测序、片段分析、dHPLC之外，还为 SNP/突变发现，焦测序SNP分型，焦测序定量CpG甲基化，以及 TaqMan SNP打字。现在还提供了实时聚合酶链式反应。在上次CCSG审查时，这 设施提供Affymetrix服务，并有一个单独的cDNA打印设施。随着时间的推移 随着微阵列技术的发展，人们的重点已经转移到了商业上 微阵列。为了满足表观遗传学研究的需要，与NimbleGen Direct签订了服务合同，以 在其位点上进行基因表达、CGH、染色质免疫沉淀在微阵列上(ChlP-ChIP)， 以及AECC开发的一种新的全基因组胞嘧啶甲基化分析。NimbleGen服务包括 由这个机构管理。在本申请的上下文中，所有微阵列服务(Affymetrix， NimbleGen和内部打印)现已整合到此共享资源中，以提供单一 为用户提供智力指导，提高成本效益。该设施提供三个通道 装有设计和分析软件的计算机工作站，以支持多项服务。在过去的时间里 五年来，该设施的产能已经增加，因此周转时间大大缩短 减少了，向用户收取的费用也减少了。

The AECC DMA Sequencing Shared Resource was established in 1992 and has evolved considerably since then and, in particular, over the last funding period, to meet the evolving scientific needs of AECC members and programs. The new name of this facility, Genomics Shared Resource, reflects this expansion of services and technologies. The facility now offers, in addition to DMA sequencing, fragment analysis, dHPLC for SNP/mutation discovery, Pyrosequencing SNP typing, pyrosequencing quantitative CpG methylation, and Taqman SNP typing. Real-time PCR is now provided as well. At the time of the last CCSG review, this Facility provided Affymetrix services, and there was a separate cDNA printing facility. With the further development of microarray technologies, there has been a shift of emphasis to commercially available microarrays. To meet the needs for epigenetic studies, services were contracted with NimbleGen Direct to perform, on their site, gene expression, CGH, chromatin immunoprecipitation on microarrays (ChlP-chip), and a novel genome-wide cytosine methylation assay developed at AECC. NimbleGen services are managed by this facility. Within the context of this application all of the microarray services (Affymetrix, NimbleGen and in-house printing) have now been consolidated in this shared resource to provide a single source of intellectual guidance to users and enhance cost-effectiveness. The facility provides access to three computer workstations with design and analysis software to support a number of the services. Over the past five years the capacity of the facility has been increased so that turnaround time has been substantially decreased and fees to users have been decreased.