Low Voltage, Economical Electroporation Device

描述（由申请人提供）：电穿孔描述了将细胞暴露在高电场中导致瞬态膜渗透的过程。这一过程已在生命科学中广泛应用于核酸、离子、染料和药物的细胞内递送。目前的电穿孔技术需要专门的电源来产生成功输送应用所必需的高电场强度。我们提出了另一种新的电穿孔装置的发展受到最近工作的刺激，描述了通过微流体通道流动的细胞的电穿孔。我们的设备也将允许使用低电压，但可以进行大批量样品处理，使用标准电源，并适用于原核和真核细胞。提出的研究将确定该系统的替代用途，包括高通量无试剂细胞裂解和电场巴氏灭菌。在这项研究中开发的产品将使电穿孔技术以显著降低的成本得到广泛应用。公众与人类的相关性：拟议的研究将导致新型电穿孔装置的发展，可用于生物医学研究界，以加强对疾病过程的基本了解。该产品将对公众健康产生直接影响，使巴氏灭菌的新型、低热量和低能源使用过程成为可能。

DESCRIPTION (provided by applicant): Electroporation describes the process of exposing cells to high electrical field which leads to transient membrane permeabilization. This procedure has gained widespread use in the life sciences for intracellular delivery of nucleic acids, ions, dyes and drugs. Current electroporation techniques require specialized power supplies to create the high electrical field strengths necessary for successful delivery applications. We propose the development of an alternate new electroporation device stimulated by recent work describing electroporation of cells flowing through microfluidic channels. Our device will also permit the use of low voltages, but enable high volume sample processing, function with standard power supplies, and be applicable for prokaryotic and eukaryotic cells. The research proposed will define alternative uses for the system including high throughput reagentless cell lysis and electrical field pasteurization. The products developed during this research will enable widespread use of electroporation at significantly reduced costs. PUBLIC HUMAN RELEVANCE: The proposed research will lead to the development of novel electroporation devices that can be used by the biomedical research community to enhance the basic understanding of disease processes. This product will have a direct impact on public health by enabling a novel, low heat and energy use process for pasteurization.