Novel Therapy to Treat Otitis Media

描述（由申请人提供）：中耳炎是一种常见的儿童疾病，每年的直接费用估计超过30亿美元。中耳炎是儿科医生就诊的最常见原因，每年导致超过2000万份抗生素处方。然而，与中耳炎相关的病原体对处方抗生素的耐药性越来越强。对慢性中耳炎病例的分子检查表明存在包裹在称为生物膜的复杂微生物群落中的细菌。抗生素药物已被证明对慢性中耳炎中的生物膜感染无效。有效对抗慢性和急性中耳炎、不诱导微生物抗性并且有效对抗生物膜的疗法将是非常有益的并且具有显著的商业潜力。该项目的目标是开发一种独特的，安全的，有效的治疗急性和慢性中耳炎。原型制剂将在体外和中耳炎动物模型中进行测试。该提案的具体目标是：具体目标1。配制用于治疗中耳炎的杀碘剂。评价碘杀灵体外对嗜酸性细菌和生物膜细菌的抗菌活性。在中耳炎动物模型中证明碘杀灵的有效性和安全性。与公共卫生的相关性：中耳炎是一种常见的儿童疾病，每年的直接费用估计超过30亿美元。中耳炎是儿科医生就诊的最常见原因，每年导致超过2000万份抗生素处方。随着抗生素的广泛使用，中耳炎相关病原体对抗生素治疗的耐药性越来越强。

DESCRIPTION (provided by applicant): Otitis media is a common childhood disease with annual direct costs estimated to exceed $3 billion. Otitis media is the most frequent cause of pediatric practitioner visits and results in more than 20 million antibiotic prescriptions annually. However, the pathogens associated with otitis media have become increasingly resistant to the prescribed antibiotics. Molecular examination of chronic cases of otitis media demonstrates the presence of bacteria encased in complex microbial communities known as biofilms. Antibiotic agents have proven to be ineffective against biofilm infections seen in chronic otitis media. A therapy that is effective against both chronic and acute otitis media, that does not induce microbial resistance, and that is effective against biofilms would be highly beneficial and have significant commercial potential. The goal of this project is to develop a unique, safe, and efficacious treatment for both acute and chronic otitis media. Prototype formulations will be tested in vitro and in an animal model of otitis media. The Specific Aims of the proposal are: Specific Aim 1. Formulate Iocide for use in treating otitis media Specific Aim 2. Evaluate Iocide for antibacterial activity on planktonic and biofilm bacteria in vitro Specific Aim 3. Demonstrate the effectiveness and safety of Iocide in an animal model of otitis media. PUBLIC HEALTH RELEVANCE: Otitis media is a common childhood disease with annual direct costs estimated to exceed $3 billion. Otitis media is the most frequent cause of pediatric practitioner visits and results in more than 20 million antibiotic prescriptions annually. Consequent to the widespread use of antibiotics, the pathogens associated otitis media have become increasingly resistant to antibiotic therapy.