Core--Neuropathology

本项目的神经病理学核心项目赠款是一个实验室功能，不同于目前戴维森博士和摩尔博士的研究实验室中存在的神经组织化学和免疫组化能力。作为摩尔博士研究实验室内的神经病理学核心，这一实验室功能的正规化将集中必要的人员、设备和试剂，以便高效地制作高质量的大脑切片和染色，以便进行显微镜评估。神经病理学核心设施的总体目标是支持Davidson、Paulson、Kosik、Gonzalez-Aregre和Dauer博士在转导表达特定shRNA的病毒后对小鼠大脑进行评估。这包括 与项目调查人员就用于显微镜评估的大脑处理、制定特定的免疫染色或组织化学染色方案、制作高质量的脑切片以及在个别研究人员实验室进行评估的染色切片进行咨询。神经病理学核心工作人员和研究人员在这一过程的所有阶段都保持密切联系。因此，Core既是基于组织学的方法的研究和开发设施，也是为显微评估做好准备的脑切片的服务设施。此外，摩尔博士(委员会认证的神经病理学家)将根据每个项目的需要提供脑部切片的标准神经病理评估。后者 在对RNAi不良影响的转导大脑进行客观评估时，责任感尤为重要。该中心的主要职责是：1)完成用于组织学评估的大脑处理；2)制作专门适应每个项目需要的显微切片；3)根据每个项目的需要制作组织化学或免疫组织化学切片；4)存储未切割的组织和未染色的切片；5)根据每个项目的需要评估大脑的一般神经病变；6)开发用于个别项目的新染色方法。

The Neuropathology Core for this Program Project Grant is a laboratory function dervied from neurohistochemistry and imunostaining capabilities that currently exist in the research laboratories of Drs. Davidson and Moore. Formalization of this laboratory function as the Neuropathology Core housed within Dr. Moore's research laboratory will centralize personnel, equipment, and reagents necessary to efficiently produce high quality brain sections and stains ready for microscopic evaluaton. The Neuropathology Core facility's overall objective is to support Drs. Davidson, Paulson, Kosik, Gonzalez-Alegre, and Dauer in the evaluation of mouse brains following transduction with viruses expressing specific shRNAs. This includes consultation with Program investigators concerning the processing of brains for microscopic evaluation, development of specific immunostaining or histochemical staining protocols, production of high quality brain sections, and staining sections for evaluation in individual investigator laboratories. The Neuropathology Core staff and investigators are in close contact through all phases of this process. Thus, the Core serves as both a research and development facility for histology-based methods and a service facility to provide brain sections ready for microscopic evaluation. In addition, Dr. Moore (a board-certified neuropathologist) will provide standard neuropathologic evaluation of brain sections as needed for each project. This latter responsibility is particularly important in the objective evaluation of transduced brains for untoward effects of RNAi. The main responsibilities of the Core will be: 1) Complete the processing of brains for histologic evaluation; 2) Produce microscopic sections specifically adapted to the needs of each project; 3) Produce histochemical or immunostained slides as needed by each project; 4) Store uncut tissue and unstained sections; 5) Evaluate brains for general neuropathological changes as needed by each project; 6) Develop new staining methods for use in individual projects.