Tissue/Pathology Core

该组织/病理学核心为研究人员提供了特征良好的人类生物标本 参与这个宫颈癌孢子和其他合作研究工作。在第一次融资中 在此期间，该核心收集了来自SPORE入组的3524名患者的78，000多份个体等分试样， 宫颈癌，迄今已分发6332份。这包括来自838名患者的28，000多份等分试样 迄今为止，从一项临床试验和两项流行病学研究JHU乳腺癌和头颈癌 孢子在没有额外的费用。除了对复杂样品采集的需求不断增长外，我们的 研究者依赖于专家病理学的支持，以确保适当的组织制备和表征 对于选定的研究。该核心提供了这样的专家病理学评价标本和技术 支持.具体而言，这一核心继续：1。获得知情同意并从患者中采集标本 在不损害患者护理或保密性的情况下进行转化研究; 2.收集宫颈癌 和癌前病变，以及来自患者的正常组织，包括那些参加临床试验的患者， SPORE项目; 3.收集患者的血液、分泌物和脱落细胞（如宫颈刮片）， 包括那些参加SPORE项目临床试验的人; 4.处理和储存临床标本 遵循SOP以满足所有SPORE调查人员的要求; 5.将样本信息输入 中央数据库系统和跟踪样本分配和运输; 6.表征组织标本 关于起源部位、病理分级和分期以及肿瘤和间质组织的比例; 7.使用明确定义的机制来优先向研究者分发所需标本 约翰霍普金斯孢子内外; 8.及时提供质量控制样本 尽可能地便宜和有效; 9.组织学和病毒学分析的样本路径，例如 免疫组织化学染色、原位杂交、组织微阵列和HPV检测和分型， 免疫分型或激光捕获显微切割在按服务收费的综合癌症中心核心 设施; 10.支持免疫分析的开发和实施。样品将 使用CaTissue（CaBIG投诉数据库）进行跟踪，我们在UAB的项目可以通过网络访问， 加州大学该核心的活动将与行政/临床核心A的活动整合， 生物统计学和数据管理核心B和免疫学核心D，以确保样本和 临床信息被适当地分类和分发。

This Tissue/Pathology Core provides well-characterized human biological specimens to researchers participating in this Cervical Cancer SPORE and other collaborative research efforts. In the first funding period this core collected over 78,000 individual aliquots from over 3524 patients enrolled by the SPORE in Cervical Cancer and distributed 6332 aliquots to date. This includes over 28,000 aliquots from 838 patients to date from a clinical trial and two epidemiologic studies of the JHU Breast Cancer and Head and Neck SPOREs at no additional cost. In addition to the growing need for sophisticated sample acquisition, our investigators depend on expert pathology support to ensure proper tissue preparation and characterization for selected studies. This Core provides such expert pathologic evaluation of specimens and technical support. Specifically, this core continues to: 1. Obtain informed consent and collect specimens from patients for translational research without compromise of patient care or confidentiality; 2. Collect cervical carcinoma and pre-malignant lesions, as well as normal tissue from patients, including those enrolled in clinical trials for the SPORE projects; 3. Collect blood, secretions and exfoliated cells (e.g. cervical scrapes) from patients, including those enrolled in clinical trials for the SPORE projects; 4. Process and store clinical specimens following SOPs to address the requirements of all SPORE investigators; 5. Input specimen information into central database system and track specimen distribution and transport; 6. Characterize tissue specimens with respect to site of origin, pathologic grading and staging, and proportion of neoplastic and stromal tissue; 7. Use well-defined mechanisms for prioritization of the distribution of requested specimens to investigators within and external to the Johns Hopkins SPORE; 8. Provide quality-controlled specimens in a timely fashion as inexpensively and efficiently as possible; 9. Route specimens for histologic and virologic analyses e.g. immunohistochemical staining, in situ hybridization, tissue microarraying, and HPV testing and typing, immunophenotyping, or laser-capture microdissection in fee-per-service Comprehensive Cancer Center core facilities; 10. Support the development and implementation of immunologic assays. The samples will be tracked using CaTissue, a CaBIG complaint database, web-enabled for access by our projects at UAB and UC. The activities of this Core will be integrated with those of the Administrative/Clinical Core A, the Biostatistics and Data Management Core B and the Immunology Core D to ensure that specimens and clinical information are appropriately catalogued and disseminated.