PROOF OF CONCEPT FOR CARD-BASED CD4 CELL COUNTING

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 这一第二阶段的SBIR应用将继续开发一种廉价的护理点卡/读卡器系统，用于监测艾滋病毒感染者的CD4+细胞，供资源匮乏地区使用。该技术使用薄膜叠层微流控盒来采集、计数和存储血样，同时存储所需的检测试剂并从台式单元注入到盒中。其目的是系统地开发该设备并对其进行故障排除，最终验证其读数，并与标准的流式细胞术进行比较，该方法使用受试者的全血来计数小体积的CD4+细胞，每次测试的费用不到1.50美元。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. This phase II SBIR application will continue the development of an inexpensive point-of-care card/reader system for monitoring CD4+ cells in HIV-infected subjects, for use in resource poor areas. The technology uses a thin-film laminated microfluidic cassette to collect, count and store the blood sample while the required detecting reagents are stored and injected into the cassettes from the bench top unit. The aims are designed to systematically develop and troubleshoot the device, ultimately validating its readings comparing with standard flow cytometry using whole blood from human subjects to enumerate CD4+ cells in small volumes for less than $1.50 per test.