GASTRIC EMPTYING AND INTESTINAL TRANSMIT TIME IN BABOONS

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 胃排空时间和小肠通过时间是评价正常胃肠功能的重要指标。 胃（胃）排空率降低或“胃排空”是2型糖尿病患者和自身免疫性疾病患者的常见发现。 本研究的目的是使用新开发的SmartPill（SmartPill Corporation，布法罗，NY）技术测量狒狒模型的胃排空和肠道通过时间。 基于人体研究，这项新技术能够以非侵入性方式提供这些参数的可靠测量。 受试者必须吞下一个小的难消化胶囊（26 x 13 mm），略大于标准的复合维生素药丸。 该胶囊采样，然后在通过肠道时将pH值，压力和温度的数据传输到移除接收器。 这项研究的目的如下： 1. 使用SmartPill技术量化狒狒摄入液体餐后的胃排空和小肠传输时间。 2. 测定狒狒液体食物胃排空和小肠传输时间的可重复性。 禁食过夜后，用氯胺酮轻度麻醉动物，以便于将口胃管置入胃中。 将通过管给予液体餐推注（Ensure，Abbott Labs），然后快速通过管给予SmartPill。 给予液体餐/SmartPill后，将对动物进行X光检查，然后将其送回各自的笼子并让其恢复。 SmartPill胶囊通过肠道的运动将由位于笼外的SmartPill数据接收器监测。 监测将在进餐后持续长达36小时（或胶囊通过所需的时间）。 SmartPill胶囊将在排泄后回收。 将在每只动物中重复该程序，以确定该技术的试验间重复性。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Gastric emptying time and intestinal transit time are important factors in the assessment of normal gastrointestinal function. A reduced rate of gastric (stomach) emptying or 'gastroparisis' is a common finding in individuals with type 2 diabetes and patients with autoimmune disease. The purpose of this study will be to use the newly developed SmartPill (SmartPill Corporation, Buffalo, NY) technology to measure gastric emptying and intestinal transit time in the baboon model. Based on human studies, this new technology is capable of providing robust measures of these parameters in a non-invasive manner. The test subject must swallow a small indigestible capsule (26 x 13mm) that is slightly larger than a standard multivitamin pill. This capsule samples and then transmits data on pH, pressure, and temperature to a remove receiver as it passes through the intestine. The aims of the study are as follows: 1. Quantify gastric emptying and small bowel transmit time following ingestion of a liquid meal in baboons using SmartPill technology. 2. Determine the repeatability of gastric emptying and small bowel transmit times for a liquid meal in baboons. After an overnight fast, animals will be lightly anesthetized with ketamine to facilitate placement of an orogastric tube into the stomach. The liquid meal bolus (Ensure, Abbott Labs) will be administered through the tube, quickly followed by administration of the SmartPill through the tube. Following liquid meal/SmartPill administration, animals will be radiographed than retuned to their individual cage and allowed to recover. Movement of the SmartPill capsule through the intestine will then be monitored by the SmartPill data receiver positioned outside the cage. Monitoring will last for up to 36 hours (or the time taken for the capsule to pass) after meal administration. The SmartPill capsule will be recoved following excretion. This procedure will be repeated in each animal to determine the test-to-test repeatability of this technique.