LOUISIANA COBRE: OBESITY & DIABETES RES: GENOMICS CORE

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 基因组学核心设施（GCF）提供DNA测序，DNA片段分析，DNA，蛋白质和RNA样品的定性和定量分析，定量PCR，微阵列RNA标记，点样，杂交和扫描，机器人和生物信息学服务。提供个人和小组培训和咨询服务，用于序列分析，实时PCR和微阵列分析。 全球气候基金的实验室服务可供任何研究人员使用。GCF的目标是以低成本提供高质量的服务，以促进研究。Genomics核心设施服务的使用按先到先得的原则安排。仪器使用的等待时间很少超过一天，通常更短。 核心实验室约900平方英尺。两个辅助实验室，每个约168平方英尺的房子微阵列杂交，扫描和微阵列点样。实验室配备有应急电源，配有专用电路用于仪器以及超纯水、气体、空气和真空供应。 实验室配备有： 两台Applied Biosystems 3130 XL 16毛细管遗传分析仪。这些仪器进行DNA测序和片段分析。它们将容纳96孔和384孔格式的样品板。 四个配备有96孔、384孔和低密度阵列块的Applied Biosystems 7900 HT序列检测系统可用于定量PCR。 Applied Biosystems 1700微阵列系统和Illumina微阵列系统可供希望使用商业微阵列系统的研究人员使用。用于蛋白质印迹的微阵列设备具有生产定制寡核苷酸或cDNA微阵列的能力。用配备有服务器臂的GeneMachine OmniGrid微阵列仪打印载玻片，并使用Perkin-Elmer ScanArray 5000扫描。使用Spotfire软件和Bioconductor R分析微阵列数据。 两个移液机器人，一个Becton Dickinson Biomek FX和一个Perkin Elmer MultiProbe II，可用于机器人液体处理。这些仪器有助于384孔板的高通量移液。它们可以编程用于大型移液项目。 Qiagen Qiacube机器人将使用Qiagen Spin柱试剂盒处理多达12个样品，用于样品分离和纯化。 两台Agilent 2100生物分析仪用于蛋白质、DNA和RNA分析和定量。 NanoDrop分光光度计用于RNA和DNA定量。 Odyssey红外成像仪使用直接红外荧光检测分析。 对于大型DNA提取和纯化项目，使用Qiagen AutoPure机器人。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The Genomics Core Facility (GCF) provides DNA sequencing, DNA fragment analysis, qualitative and quantitative analysis of DNA, protein, and RNA samples, quantitative PCR, microarray RNA labeling, spotting, hybridization, and scanning, robotics, and bioinformatics services. Individual and small group training and consultation services are offered for sequence analysis, real-time PCR, and microarray analysis. The GCF laboratory services are available to any researcher. The goal of the GCF is to provide high quality service at low cost to facilitate research. Use of the Genomics Core Facility services are scheduled on a first come, first serve basis. The wait time for instrument use is rarely more than one day and is frequently shorter. The core laboratory is approximately 900 square feet. Two ancillary laboratories of approximately 168 square feet each house microarray hybridization, scanning, and microarray spotting. Laboratories are supplied with emergency power with dedicated circuits for instrumentation as well as ultrapure water, gas, air, and vacuum supplies. The laboratory is equipped with: Two Applied Biosystems 3130XL sixteen capillary genetic analyzers. These instruments perform DNA sequencing and fragment analysis. They will accommodate 96 well and 384 well format sample plates. Four Applied Biosystems 7900HT Sequence Detection Systems equipped with 96 well, 384 well, and low density array blocks are available for quantitative PCR. An Applied Biosystems 1700 microarray system and an Illumina microarray system are available for researchers who wish to use a commercial microarray system. The microarray facilityfor western blot an has the capability to produce custom oligonucleotide or cDNA microarrays. Slides are printed with a GeneMachine OmniGrid Microarrayer equipped with server arm and scanned using a Perkin-Elmer ScanArray 5000. Spotfire software and Bioconductor R Scripts are used for analysis of microarray data. Two pipetting robots, a Becton Dickinson Biomek FX and a Perkin Elmer MultiProbe II, are available for robotic liquid handling. These instruments facilitate high-throughput pipetting of 384 well format plates. They can be programmed for large pipetting projects. A Qiagen Qiacube robot will process up to twelve samples using Qiagen Spin column kits for sample isolation and purification. Two Agilent 2100 Bioanalyzers are used for protein, DNA, and RNA analysis and quantitation. A NanoDrop Spectrophotometer is used for RNA and DNA quantification. An Odyssey Infrared Imager uses direct infrared fluorescence detection analysis. For large DNA extraction and purification projects, a Qiagen AutoPure robot is used.