MOLECULAR BIOLOGY AND BIOCHEMISTRY SUPPORT CORE

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 核心C，分子生物学和生物化学支持设施，提供必要的接口，专业知识，培训和指导基因阵列分析，以推进COBRE项目。基因阵列技术提供了一种高效的手段来筛选单个细胞类型的基因表达模式和变化。基因微阵列处理和生物信息学服务可通过K-INBRE NIH赠款的赞助获得，从而使我们能够将核心支持集中在样品制备（质量和数量分析），KSU和KUMC其他设施的基因微阵列处理资金以及后基因微阵列验证（qRT-PCR）。Core通过将研究者RNA样本外包给其他机构（堪萨斯州立大学和堪萨斯大学医学中心）以进行基因芯片制备和杂交来提供基因阵列支持;此外，COBRE Core Manager和上述服务提供处理基因阵列数据的生物信息学支持。Core通过以下方式提供支持：1）三个实时聚合酶链反应（PCR）热循环仪，用于定量评估基因转录本表达; 2）Agilent BioAnalyzer，用于确定基因阵列分析所获得的RNA样品的质量; 3）Nanodrop分光光度计和荧光分光光度计，用于确定核苷酸和蛋白质样品的数量;以及4）DNA测序仪，用于验证PCR产物的同一性。需要实时PCR来验证基因阵列结果或比基因阵列更详细地分析特定基因表达变化。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Core C, the Molecular Biology and Biochemistry Support facility, provides the interface, expertise, training and mentoring in gene array analysis necessary to advance COBRE projects. Gene array technology provides a highly efficient means to screen individual cell types for gene expression patterns and changes. Gene microarray processing and bioinformatics services are available through the auspices of a K-INBRE NIH grant, thereby allowing us to focus Core support on sample preparation (analyses of quality and quantity), funding of gene microarray processing by other facilities at KSU and KUMC and of post-gene microarray validation (qRT-PCR). The Core provides gene array support by outsourcing to other facilities (at Kansas State University and Kansas University Medical Center) investigator RNA samples for gene chip preparation and hybridization; in addition, bioinformatics support to process the gene array data is provided by the COBRE Core Manager and by the service referenced above. The Core provides support through: 1) three, real-time polymerase chain reaction (PCR) thermocyclers for quantitative assessment of gene transcript expression, 2) an Agilent BioAnalyzer for determining the quality of RNA samples obtained for gene array analysis, 3) a Nanodrop spectrophotometer and spectrofluorometer for determining the quantity of nucleotide and protein samples, and 4) a DNA sequencer for verifying PCR product identity. The real-time PCR is needed to verify gene array results or analyze specific gene expression changes in more detail than is possible with the gene array.