ANALYSIS OF MITOCHONDRIAL POOLS OF IRON BY XAS

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 最近的遗传学实验表明，酵母的有丝分裂膜中存在前所未有的铁，尤其是为线粒体中存在两种不同的铁池提供了证据。这种形态会影响线粒体超氧化物歧化酶(SOD)的活性，因为一个铁库中的铁能够取代酶的活性形式中的锰，而另一个铁库中的铁是SOD-惰性的。WT的XAS和各种突变的酵母线粒体将被用来为这两个线粒体铁池提供详细的局部结构信息。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Recent genetic experiments have suggested an unprecedented speciation of iron within the mitochrondia of yeast, and in particular have provided evidence for two different pools of iron in mitochondria. This speciation effects the activity of mitochondrial superoxide dismutase (SOD), as iron from one pool is able to displace manganese from the active form of the enzyme, with the other iron pool is SOD-inert. XAS of WT and various mutant yeast mitochondria will be used to provide detailed local structural information for these two pools of mitochrondial iron.