TRANSIENT STRUCTURAL STATES OF GROEL

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 摘要 一个合作项目(Art Horwich&George Farr，耶鲁大学)已经开始研究伴侣蛋白GroEL的瞬时结构状态。该项目将利用时间分辨低温电磁的预混合/喷雾和闪光光解模式。我们已经在以前的报告中描述了我们实现的闪光光解。一个尚未完全解决的问题是，当暴露在紫外光闪光下时，反应溶液的温度会上升到什么程度，这会引发笼状反应物的光解。目前，为了将温度升高降至最低，我们使用50微米厚的铜栅(由LADD Research定制)，在铜栅上涂覆铝，然后覆盖碳网，以最大限度地减少碳对光子的吸收。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. ABSTRACT A collaborative project (Art Horwich & George Farr, Yale) to investigate transient structural states of the chaperonin, GroEL, has commenced. This project will make use of the pre-mix/spray and flash-photolysis modes of time-resolved cryo-EM. We have described our implementation of flash-photolysis in previous reports. One problem that remains not fully resolved is the degree to which the temperature of the reaction solution rises upon exposure to a UV light flash which initiates photolysis of caged reactants. Currently, to minimize the temperature rise we use 50-um thick copper grids (custom made by Ladd Research) that are coated with aluminum and then covered with carbon nets to minimize absorption of photons by the carbon.