HIGH THROUGHPUT STRUCTURE DETERMINATION OF PROTEIN COMPLEXES

这个子项目是许多利用 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 新世纪制药公司利用NSLS同步加速器进行高通量的蛋白质复合物晶体结构测定。天然蛋白质的结构是已知的。由于晶体的空间群（三斜晶系），在理学/MSA内部X射线设备上收集一个数据集大约需要3天。大量的复合物迅速饱和的能力，我们的家庭来源。使用NSLS光束线，我们希望在X射线数据收集上有更快的周转。这反过来又将显着增加吞吐量，加快研究过程和合理的药物设计工作。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. New Century Pharmaceuticals, Inc. is going to conduct high throughput crystal structure determination of protien-compound complexes using NSLS synchrotron facility. The strucutre of the native protein is already known. Due to the space group of the crystals (triclinic), it takes about 3 days to collect one dataset on the in-house Rigaku/MSA x-ray equipment. Large number of complexes quickly saturates the capacity of our home sources. Using NSLS beamlines, we are hoping to have quicker turnaround on X-ray data collection. This in turn will significantly increase the throughput and spped up the research process and rational drug design efforts.