SCREENING FOR BXI1P (BAX INHIBITOR-1) INTERACTING PROTEINS

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 Bax抑制因子-1(BI-1)首先被鉴定为能够阻断Bax诱导的酿酒酵母(Saccharmyces Cerevisiae)细胞凋亡。它是调控细胞程序性死亡的Bcl2蛋白家族的成员之一。有趣的是，BI-1是在广泛的真菌、植物和动物物种中发现的为数不多的细胞死亡抑制剂之一，包括酿酒酵母、黑腹果蝇、拟南芥、小鼠和智人。这表明它是一种古老的蛋白质。我们正在鉴定BI-1的酵母同源物，我们称之为BXI1。使用双杂交筛选，我们希望识别与Bxi1p相互作用的蛋白质。随着对BXI1在酵母中功能的了解，我们希望更好地了解Bax抑制物-1在包括人类在内的高等生物中的功能。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Bax Inhibitor-1 (BI-1) was first identified by its ability to block Bax-induced apoptotic cell death in the budding yeast, Saccharomyces cerevisiae. It is one member of the Bcl-2 family of proteins that regulates programmed cell death. Intriguingly, BI-1 is one of a few cell death inhibitors found in a wide range of fungi, plant, and animal species including Saccharomyces cerevisiae, Drosophila melanogaster, Arabidopsis thaliana, Mus musculus, and Homo sapiens. This suggests that it is an ancient protein. We are characterizing the yeast homolog of BI-1, which we are calling BXI1. Using a two-hybrid screen, we would like to identify proteins that interact with Bxi1p. With knowledge of the function of BXI1 in yeast we hope to better understand the function of Bax Inhibitor-1 in higher organisms including human beings.