RECONSTRUCTION AND MODELING OF NORMAL AND GENETICALLY ENGINEERED MOUSE HEART

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 利用共聚焦显微镜在亚微米分辨率下对整个小鼠心脏进行三维重建是非常重要的。图像收集和重建正在对正常和基因改变小鼠的心脏进行。关于异质分布的组织特性和整体心室结构的信息将用于创建用于小鼠心脏的完全耦合机电模拟的有限元网格。 这些模拟将使我们能够预测特定遗传操作对心脏整体机械和电功能的影响。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. It is important to generate three-dimensional reconstructions of whole mouse hearts at sub-micron resolution using confocal microscopy. Image collection and reconstruction is being performed on hearts from both normal and genetically altered mice. This information on heterogeneously distributed tissue properties and overall ventricular structure will be used to create finite element meshes for fully-coupled electromechanical simulations of the mouse heart. These simulations will allow us to predict the effects of specific genetic manipulations on the overall mechanical and electrical function of the heart.